摘要
目的观察钙网蛋白(CRT)在瘢痕及正常皮肤成纤维细胞内的表达情况。方法采用免疫细胞化学染色和原位 ELISA 技术对体外培养的瘢痕及正常皮肤成纤维细胞进行 CRT 分布定位及表达水平研究。结果瘢痕和正常皮肤成纤维细胞在处于增殖生长状态时,胞浆及核内均有不同程度的 CRT 表达,其中瘢痕成纤维细胞表达 CRT 水平(A:1.97±0.15,ELISA492nm)明显高于正常皮肤成纤维细胞水平(A:1.43±0.12),差异有非常显著意义(P<0.01);而在已融合的瘢痕和正常皮肤成纤维细胞内则均无 CRT 表达。结论瘢痕成纤维细胞内 CRT 的丰富表达对促进细胞迁移、信号传递及钙存储等方面均有着积极作用。
Objective To investigate the expression level and intracellular distribution of calretic- ulin(CRT)in hypertrophic scar-derived fibroblasts(HSF)and normal skin-derived fibroblasts(NSF). Methods Using anti-CRT antibody,CRT was detected in HSF and NSF by immunocytochemistry and ELISA technique.Results CRT was present in the cytoplasm and nuclei in proliferating HSF or NSF in culture,but HSF possessed significantly higher expression level of CRT than NSF(P<0.01).When these cells(HSF and NSF)were fused,neither intracellular nor intranuclear staining for CRT was ob- served.Conclusion In HSF and NSF,the distribution of CRT is widespread.Because of its abundant ex- pression in HSF,we postulate that CRT as a multifunctional protein plays an important role in Ca^(2+) se- questering,integrin-mediated signalling and cell adhesion.
关键词
瘢痕
成纤维细胞
钙网蛋白
表达
Hypertrophic scar
Fibroblast
Calreticulin
Expression
