牛透明软骨细胞与组织引导再生胶原膜体外培养后植入体内的研究

A study on guided tissue regeneration and tissue engineering-transplantation of collagen membrane seeded with cultured hyaline chondrocytes

目的解决修复重建组织器官缺损所需移植材料的问题,利用组织工程技术,进行组织工程牛透明软骨的实验研究。方法用分离获得的牛软骨细胞,与医用组织引导再生胶原膜体外联合培养1周后,以软骨细胞可降解材料复合物的形式植入裸鼠体内,8周后对所获得的组织工程软骨样组织进行形态学研究。结果在实验获得的组织工程软骨样组织中有软骨细胞的存在,并且可以分泌硫酸软骨素。证实为组织工程牛透明软骨。结论实验结果表明应用组织工程技术,在体外进行组织工程软骨的制作,可能成为解决整形外科领域修复缺损用移植材料不足的方法之一。

Objective Lack of transplant material is a main problem in the repair and reconstruc- tion of tissue or organ defects.To seek the succedaneum of transplants,we studied and used tissue-engi- neered growth hyaline cartilage.Methods The articular hyaline cartilage obtained from newborn calf within 6 hours of sacrifice was enzymatically dissolved.The cbondrocytes were then seeded onto the medi- cal collagen membrane of guided tissue regeneration in a 24 well plate in RPMI1640 medium.One week later,the chondrocyte medical collagen membrane of guided tissue regeneration complex was implanted subcutaneously into the back of nude mice.Eight weeks after implantation,the animals were sacrificed. Results The present study seems to demonstrate that the cartilage-like tissue was strong enough to be transferred after being implanted for 8 weeks.The cartilage-like tissue was proved to be tissue-engineered hyaline cartilage by HE stain.The chondrocytes could secrete chondroitin sulfate as proved by Lev-Spicer stain.Conclusion Tissue engineering technique was used to make tissue-engineered cartilage in vitro.It will provide a new transplant material for the repair of tissue or organ defects.