重金属镉与铜在棕尾别麻蝇体内的积累和排泄研究

Study on accumulation and excretion of dietary cadmium and copper by Boettcherisca peregrine (Diptera:Sarcophagidae)

为了研究镉(Cadmium,Cd)和铜(Copper,Cu)在棕尾别麻蝇Boettcherisca peregrina(Diptera:Sarcophagidae)生长发育过程中积累和排泄的动态变化,在室内给棕尾别麻蝇初产幼虫连续饲喂含不同浓度Cd2+或Cu2+的人工饲料,每隔24h测定幼虫对Cd2+或Cu2+积累和排泄及变态过程中的变化。结果表明:棕尾别麻蝇幼虫取食含有Cd2+的饲料后,大部分Cd2+积累在体内,少部分通过排泄系统排出。Cd2+在幼虫体内的积累量随着取食时间的延长而增加,幼虫取食含200μg/gCd2+饲料后,体内Cd2+积累量高于取食含100μg/g、400μg/g和800μg/gCd2+饲料后体内Cd2+积累量,幼虫对Cd2+排泄量亦有相同的规律。Cd2+在幼虫、蛹和刚羽化成虫中的含量依次降低。棕尾别麻蝇幼虫取食含Cu2+的饲料后,其体内Cu2+积累量小于排泄量,而且体内积累量和排泄量随着取食时间的延长而增加,Cu2+积累量和排泄量与取食饲料中的Cu2+浓度均不成正比。在变态过程中,Cu2+也不断被排泄,体内Cu2+含量随之下降。

For studying the dynamical variations of accumulation and excretion of cadmium and copper in Boettcherisca peregrine （Diptera：Sarcophagidae） during growth and development,newly oviposited larvae of the fly were exposed to different concentrations of Cd2＋ or Cu2＋ administered in the artificial diet under laboratory condition,and the accumulation and excretion of Cd2＋ or Cu2＋ by the larvae every 24 h and changes in the metamorphosis were measured. The results showed that the larvae fed on diets supplemented with Cd2＋ retained most of ingested Cd2＋, only few ingested Cd2＋ were excreted by excretory system. Cd2＋ accumulation in larvae of B. peregrina increased with the prolonging of feeding time, but was not proportional to Cd2＋ concentration in diets. Cd2＋ accumulation in larvae at 200 μg/g exposure was higher than that in larvae at 100 μg/g, 400 μg/g and 800 μg/g Cd2＋ exposure. Cd2＋ excretion of the larvae showed the same tendency. During metamorphosis, Cd2＋ contents in the pupae was lower than that of in the larvae, newly eclosion adults had lower Cd2＋ contents than the larvae. After B. peregrina larvae fed on diets supplemented with different concentrations of Cu2＋, Cu2＋ contents retained in the larval body were less than that excreted by excretory system. Cu2＋ accumulation and excretion of B. peregrina larvae increased with the prolonging of feeding time, and Cu2＋ accumulation and excretion were not in direct proportion to Cu2＋ concentrations administered in the artificial diet. During metamorphosis, Cu2＋ was excreted continually and Cu2＋ content in vivo decreased.