DEAE-纤维素分离提纯海带硫酸多糖的研究

Study on the Separation and Purification of Polysaccharide Sulfate in Laminaria Using DEAE-Cellulose

采用微波提取法对两种海带样品多糖成分进行提取,通过DEAE-纤维素离子交换柱分离法纯化。结果表明:柱分离时,洗脱流速保持在1.0～1.5mL/min之间,洗脱液为0.04mol/L、0.05mol/L、0.06mol/LNaOH时,两种海带多糖可分别分离纯化出两种硫酸多糖:A1、A2和J1、J2。用硫酸钡浊度法测得硫酸根的含量分别为:6.26%、3.69%、1.02%、3.09%。

For two laminaria samples, polysaccharide was extracted by microwave-assiated extraction method, and purificated by DEAE-cellulose ion exchange column. The results indicated that distilling speed was 1.0-1 .5 mL/min, when the distill solid was 0.04 mol/L, 0.05 mol/L, 0.06 mol/L NaOH, two polysaceharides sulfates in these two sample could be purificated and separetely as： A1, A2, J1, J2. The content of sulfate ion was determinated by barium sulfate turbidimetry method, the result was 6.26 %, 3.69 %, 1.02 %, 3.09 %.