摘要
对共价闭合环状脱氧核糖核酸的检测已成为乙型肝炎患者的临床药物试验研究及病理诊断的重要检验方法。目前,普遍采用三种对共价闭合环状脱氧核糖核酸定量检测的方法:选择性聚合酶链反应法,侵入者探针法和嵌合引物荧光聚合酶链反应法。每种方法均有各自的原理和优缺点,同时对提高检测的特异性,避免假阳性发生等方法学上的内容有共同点。由于目前尚未标准化,临床需根据实际选择合适的方法,使检查在操作,结果和价格上达到最大的优化。
Detected covalently closed circular HBV DNA has on clinical trials involving patients suffering from HBV infection in addition to clinical trials of pathological diagnosis.At present,three types of quantitative detection measures are widely used for covalently closed circular DNA,namely selective PCR,invader probe and chimeric primer fluorescent PCR.Each method has its own merits and weakness,improving the specificity to avoid the ocurrence of false positive methodological content in common.It is very important to choose one of the most suitable measurements according to clinical demands while a more ideal,simple and economic test is in the investigation.
出处
《医学综述》
2010年第18期2849-2852,共4页
Medical Recapitulate
基金
南京军区"十一五"重点课题(06Z011)
