摘要
以含水乙醇为溶剂,在单因素试验基础上,采用响应曲面法优化提取桑叶黄酮工艺条件。结果表明,醇法提取桑叶黄酮的最优工艺条件为:乙醇体积分数71.75%、提取温度67.1℃、料液比23.2:1(醇溶液:桑叶粉,mL:g)、提取时间150 min、提取次数2,桑叶黄酮得率为2.37%。验证试验表明,该优化工艺稳定,与模型预测值相符。
On the basis of single factor experiment,response surface methodology（RSM） was applied to optimize extraction of flavonoids from mulberry leaves using alcoholic solution.The results showed that the optimized extraction conditions were 71.75% of ethanol,extraction temperature at 67.1 ℃,ratio of solvent to material of 23.2：1（mL·g^-1）,extraction time of 150 min and extracted two times.Under these extraction conditions,the yield of flavoniods reached at 2.37%,which agreed with the predicted value and indicated the suitability of RSM in optimizing the extraction of flavonoids from mulberry leaves.
出处
《氨基酸和生物资源》
CAS
2010年第3期76-79,86,共5页
Amino Acids & Biotic Resources
基金
浙江省科技厅项目(2007C12019)
浙江省教育厅科研基金(Y200803745)
关键词
桑叶
黄酮
响应曲面法
提取工艺
mulberry leaves
flavonoids
response surface methodology
extraction
