枯草芽孢杆菌BAB-1产脂肽类及挥发性物质的分离和鉴定

Isolation and Identification of Lipopeptides and Volatile Compounds Produced by Bacillus subtilis Strain BAB-1

【目的】对枯草芽孢杆菌Bacillus subtilis菌株BAB-1产生的脂肽类物质和挥发性物质进行分离鉴定和抑菌活性研究。【方法】采用HPLC和MALDI-TOF-MS对脂肽类物质进行分离纯化和鉴定;采用GC-MS对挥发性物质进行鉴定。【结果】HPLC分析表明,菌株BAB-1产生的脂肽类物质由LP-1、LP-2、LP-3三类组分组成,其中LP-2起主要抑菌作用。MALDI-TOF-MS质谱分析表明,LP-1为surfactin(C13—C15),LP-2为fengycin A(C14—C15)和fengycin B(C14—C18),LP-3成分未能确定。GC-MS结果表明,菌株BAB-1产生的挥发性物质由多种成分组成,主要为醇类、酮类、酸类、胺类和烷烃类等。【结论】枯草芽孢杆菌菌株BAB-1能够产生脂肽类物质和挥发性物质,并且这两类物质都具有较强的抑菌活性。

【Objective】 The objective of this study was to isolate and identify the lipopeptides and volatile compounds produced by Bacillus subtilis strain BAB-1,and to analyze their antifungal activities.【Method】 Lipopepetide compounds were isolated,purified and identified by using HPLC and MALDI-TOF-MS techniques,and the volatile compounds（VCs） were identified by using GC-MS method.【Result】 Three fractions of the lipopeptides,LP-1,LP-2 and LP-3,were collected,among which LP-2 showed the major antifungal activity.According to the mass spectra analysis,LP-1 was determined to be identical to surfactin,varying in the length of the fatty acid chain moiety from C13 to C15.LP-2 consisted of fengycin A（C14—C15） and fengycin B（C14—C18）,and LP-3 remained undetermined.Many different kinds of VCs were identified,such as alcohols,ketones,acids,amines,alkanes and so on.【Conclusion】 B.subtilis strain BAB-1 can produce lipopeptides and VCs both with strong antifungal activities.