体外法研究不同油脂对瘤胃原虫吞噬细菌微循环的影响

Effects of Different Oils on Bacteria Recycling Due to Predation by Rumen Protozoa in vitro

【目的】研究体外培养条件下,不同油脂对瘤胃原虫吞噬细菌微循环及微生物群体的影响。【方法】3头瘤胃瘘管山羊提供瘤胃液,分别以菜籽油(A)、豆油(B)、玉米油(C)和花生油(D)为底物培养瘤胃微生物,同时设空白对照组,培养10h时用荧光标记瘤胃细菌法进行原虫吞噬细菌试验。【结果】原虫密度以对照组最高,显著高于A、B、C组(P<0.05);细菌密度以B组最高,为4.906×109cells·mL-1,显著高于对照组(P<0.05);原虫蛋白在组间差异不显著(P>0.05),细菌蛋白组以B组最高,B、C、D均高于对照组(P<0.05)。各组原虫的吞噬速率分别为:267.6(对照组)、196.2(A组)、244.4(B组)、274.4(C组)、285.4(D组)cells/(cell·h),组间差异显著(P<0.05)。吞噬细菌N的速率分别为1.445、1.059、0.320、1.482、1.541pgN·(cell·h)-1。【结论】估算每天每头山羊细菌蛋白循环量分别为121.64(对照组)、56.64(A组)、78.39(B组)、87.04(C组)和95.06(D组)mg蛋白·(d·头)-1,B组较低,仅为对照组的64.45%,且该组微生物量最大。在本试验的基础上认为4%的豆油对微循环的调控效应最好。

Objective The main objectives of the experiment were to determine the effects of different oils on the bacterial recycling due to predation by rumen protozoa in vitro.Method Three Xuhuai White Goats fitted with cannulas were used to provide rumen liquid.An one-factor experiment was carried out,and the groups were divided into control group,rapeseed oil（A）,soybean oil（B）,corn oil（C）,and peanut oil（D）,respectively.The fluorescence-labeled technique was introduced to investigate the protozoal grazing rate at 10 h after in vitro culture.Result The results showed that,protozoal densities was the highest in the control group,significantly higher than that of A,B,and C（P0.05）.Bacteria density was the highest in B（4.906×109cells·mL-1）,and higher than that of control group significantly（P0.05）.No significant diference was detected in protozoal protein（P0.05）,while significant diference was found in bacterial protein,with the highest data falling in group B,and the bacterial protein of B,C,and D were higher than that of control group（P0.05）.The grazing rates of protozoa were：267.6,196.2,244.4,274.4,and 285.4 cells/（cell·h）,for control group,A,B,C,and D,respectively.There were significant differences in protozoal grazing rate among groups（P0.05）.When converted to the predation rates of bacterial N,there were 1.445,1.059,0.320,1.482,and 1.541 pg N·（cell·h）-1,respectively.Conclusion The estimation of the recycling quantity of bacterial protein by protozoa per goats per day,respectively,were 121.64,56.64,78.39,87.04,and 95.06 mg Pr·（d·head）-1,with B showing a much lower data,just accounting for 64.45% of the control group.And B held the highest microbial protein,thus,B could regulate microbial protein recycling in some degree.