毛冬青药材中毛冬青皂苷甲和毛冬青皂苷B_2的含量测定的研究

Determination of Ilexsaponin A1and IlexsaponinB_2 Contents in Ilex pubescens by HPLC-ELSD

目的建立毛冬青药材的含量测定方法。方法采用Lichrospher-C18色谱柱(4.0 mm×250 mm,5μm),流动相为乙腈-0.1%醋酸溶液(37∶63),流速1 ml/min,蒸发光散射检测器:漂移管温度107℃,气体流速2.6 L/min。结果毛冬青皂苷甲在5.19～64.88μg浓度范围内线性关系良好,r=0.999 8(n=6),平均回收率为100.26%,RSD=1.09%(n=6);毛冬青皂苷B2在0.9～11.25μg浓度范围内线性关系良好,r=0.999 7(n=6),平均回收率为101.39%,RSD=1.46%(n=6)。结论该法操作快速、准确,可作为毛冬青药材的质量控制方法。

Objective To establish an HPLC-ELSD method for the determination of Ilexsaponin A1 and Ilexsaponin B2 contents in Ilex Pubescens.MethodsLichroshper-C18（4.0 mm×250 mm,5 μm） column was used in HPLC with An evaporative light-scattering detector.Mobile phase was Acetonitrite-0.1%Acetic acid（37∶63）,the flow rate was 1 ml/min： drift tube temperature was 107℃,and gas flow rate was 2.6 L/min.ResultsThe linear range of ilexsaponin A1 was within 5.19 ～ 64.88 μg,r=0.999 8（n=6）.The average recovery was 100.26%,RSD=1.09%（n=6）.The linear range of ilexsaponin B2 was within 0.9 ～ 11.25 μg,r=0.999 7（n=6）.The average recovery was 101.39%,RSD=1.46%（n=6）.ConclusionThis method is rapid and accurate.It can be used for quality control of Ilex Pubescens.