胆固醇、甘油三酯、高密度脂蛋白与红细胞膜离子酶活性关系研究

Plasma Cholesterol,Triglycerides,High Density Lipoprotein,and Erythrocyte Membrane Ion Transport Enzymes

目的探讨高血压时血浆胆固醇（Ｃｈｏ）、甘油三酯（ＴＧ）和高密度脂蛋白（ＨＤＬ－Ｃ）水平与细胞膜离子运输酶活性之间的关系。方法３２名正常人（ＮＴ），５５例原发性高血压（ＨＴ）患者，检测血浆Ｃｈｏ、ＴＧ、ＨＤＬ－Ｃ水平，红细胞膜Ｎａ＋－Ｋ＋－ＡＴＰ酶和Ｃａ２＋－ＡＴＰ酶活性，膜Ｃ／Ｐ克分子比率及膜内面Ｃａ２＋结合力。结果（１）ＨＴ组平均动脉血压（ＭＡＰ）与ＮＴ组相比有显著性差别；（２）ＨＴ组血浆ＨＤＬ－Ｃ水平、红细胞膜Ｎａ＋－Ｋ＋－ＡＴＰ酶和Ｃａ２＋－ＡＴＰ酶活性，以及膜内面Ｃａ２＋结合力均较ＮＴ组明显减低；（３）ＨＴ组血浆Ｃｈｏ，ＴＧ水平、红细胞膜Ｃｈｏ含量和Ｃ／Ｐ克分子比率，与ＮＴ组比较皆明显增高。结论红细胞膜Ｎａ＋－Ｋ＋－ＡＴＰ酶和Ｃａ２＋－ＡＴＰ酶活性减低，以及膜内面Ｃａ２＋结合力减低，是高血压时细胞膜离子运输失常的主要标志，血浆ＴＧ水平升高和膜磷脂水平减低可能是高血压时细胞膜理化特性及离子运输失常的主要决定因素。

Aim \ To investigate the relationship between plasma cholesterol(Cho), triglycerides(TG)high density lipoprotein(HDL) and ion transport enzymes activities of red cell membranes in patients with essential hypertension.\ Methods \ Plasma ChTG,HDLC,activities of Na+K+ATPase and Ca2+ATPase,Ca2+binding capacity of membrane innersurface and membrane Cho, phospholipids(PL) were measured in 32 normotensive (NT) subjects, 55 patients with essential hypertension.\ Results \ (1)Mean artery pressure(MAP), plasma ChTG and membrane Cho levels, and membrane cholesterol/phospholipids(C/P)molar ratio are significantly increased when compared with those in NT group; (2)The plasma HDLC level and activities of Na+K+ATPase and Ca2+ATPase, and the inner surface Ca2+binding capacity in HT group are significantly decreased than those in NT group,respectively.\ Conclusion \ The depressed activities of Na+K+ATPase and Ca2+ATPase, the decreased innersurface Ca2+binding capacity of cell membranes are the major ions transport abnormalities in essential hypertension. The plasma TG and membrane C/P molar ratiodependent changes in membrane microviscosity seems to be responsible for the modulation of particular ion transport pathways.