摘要
目的旨在观察Ｌ－苯丙氨酸对自发性高血压大鼠心肌胶原组织的影响。方法：８只断奶雄性自发性血压大鼠随机分为两组。实验组饲以含３％Ｌ－苯丙氨酸的大鼠饲料，对照组饲以标准饲料，共８周。以大鼠心肌组织的总ｍＲＮＡ为实验材料，以大鼠Ｉ型胶原α２链［α２（Ｉ）］的ｃＤＮＡ探针进行Ｎｏｒｔｈｅｒｎ杂交分析。结果实验组的α２（Ｉ）ｍＲＮＡ表达强度较对照组明显减低。结论Ｌ－苯丙氨酸抑制ＳＨＲ心肌组织α２（Ｉ）ｍＲＮＡ表达，可能是Ｌ－苯丙氨酸预防ＳＨＲ心肌肥厚的机理所在。
Objective \ The purpose of the study was to investigate the effect of LPhenylalanine(Phe) on expression of 2 chain mRNA of collagen type I in myocardium of spontaneously hypertensive rat(SHR).\ Methods \ 8 weanling male SHRs were randomly divided into experimental and control groups.The former was fed with chow supplemented 3% Phe, the latter with standard rat chow in standard condition for 8 weeks. Total mRNA from SHR myocardium of the two groups was extracted and then Northern blot analysis was performed with probe of 2(I) cDNA and actin as inner standard.\ Results \ The exprssion level of 2(I)mRNA was reduced significantly in experimental group. \ Conclusion \ LPhe might inhibited the expression of 2(I) mRNA in SHR, which may be the mechanism of Phe.preventing myocardium hypertrophy of SHR.\=
出处
《高血压杂志》
CSCD
1999年第2期165-167,共3页
Chinese Journal of Hypertension
基金
国家自然科学基金
关键词
L-苯丙氨酸
高血压
胶原
心肌
治疗
LPhenylalanine
\ SHR
\ Collagen
\ myocardium
\ Northern blot analysis\=\=
