T型和L型钙通道阻断剂改善大鼠糖尿病肾功与糖、脂代谢无关

No Relation of Carbohydrate and Lipid Metamolism in Ameliorating of Renal Function by TType and LType Calcium Channel Blockage \;on Diabetic Rats

目的阐明Ｔ通道阻滞剂米贝地尔和Ｌ通道阻滞剂氨氯地平对实验性糖尿病鼠肾的保护作用是否基于改善糖、脂水平。方法雄性ＳＤ大鼠右肾切除，给予链脲佐菌素（ＳＴＺ）按６５ｍｇ／ｋｇ腹腔注射。米贝地尔组（Ｍｉｂ，３０ｍｇ／ｋｇ，ｑｄ，ｎ＝６）；氨氯地平组（Ａｍｌ，...

Objectives \ To examine whether the antiproteinnuric effects of Ttype and Ltype calcium channel antagonists in streptozotocin(STZ)induced diabetic rat is related to the ameliraation of glucose and lipid metabolism or not.\ Methods \ Two weeks after being uninephrectomized (UNx), male SD rats were insulted to have diabetes by STZ. Ttype calcium channel antagonist mibefradil (30 mg/kg),Ltype calcium channel antagonist amlodipine (30 mg/kg), angiotensin converting enzymeinhibitor(ACEI) cilazapril(10 mg/kg) were given respectively by daily gavage for 4 weeks from the next day of STZ injection. Another two groups, untreated uninephrectomized diabetic group and uninephrectomized group were served as Author control. Blood pressure was measured weekly by tailcuff method. 24 h urine was collected in a metabolic cage to determine proteinuria and creatinine clearance rate(Ccr). Plasma glucose, triglyceride and cholesterol concentration as well as plasma ACE activity, serum insulin and Cpeptide, HbA1c were measured.\ Results \ All diabetic rats showed overt proteinuria, increased Ccr, glucoselipid, insulin, Cpeptide and HbA1c. Treated animals presented less urinary protein and less elevated Ccr. Although cilazapril group had some decreased in blood glucose and serum cholesterone, no any changes were detected in mibefradil and amlodipine treated groups. No remarkable differences in plasma insulin, Cpeptide and HbA1c levels were found among nontreated and treated diabetes.\ ConclusionBZ]\ The protective effect of calcium channel blockage on diabetic nephropathy might not mediated by improvement of glucose and lipid metabolism.