摘要
目的研究ＯＸ－ＬＤＬ及抗氧化剂对平滑肌细胞信号转导的影响。方法采用薄层层析、放射自显影及放射酶标法分离、检测细胞中ＤＡＧ含量。结果ＯＸ－ＬＤＬ刺激平滑肌细胞产生ＤＡＧ具有双时相性变化，并且随浓度增加而增加。抗氧化剂ＶｉｔＥ明显抑制平滑肌细胞产生ＤＡＧ。结论ＯＸ－ＬＤＬ能明显刺激ＤＡＧ的生成，抗氧化剂ＶｉｔＥ显著抑制平滑肌细胞ＤＡＧ的形成，并且具有浓度依赖效应。
Aim \ To study the effect of oxidized low density
lipoprotein (OXLDL) and antioxidant on cell signal transduction in cultured smooth muscle cells.
\ Methods \ Separation and quantitative measurement of diacylglycerol (DAG) were studied
using thinlayer chromatography, autoradiography and radioenzymatic assay.\ Results \ The
DAG levels in ASMC were increased by OXLDL in a dosedependent, biphasic manners.
Antioxidant VitE can markedly inhibit the OXLDLstimulated formation of DAG in ASMC.\
Conclusion \ OXLDL causes a robust stimulation of DAGPKC pathway in cultured smooth muscle
cells .VitE can significantly attenuate the accumulation of DAG in a concentrationdependent
manner.
出处
《高血压杂志》
CSCD
1999年第2期181-183,共3页
Chinese Journal of Hypertension
关键词
动脉粥样硬化
氧化LDL
抗氧化剂
二酰基甘油
oxidized low density lipoproteins
\ smooth muscle cultured cells
\
Diacylglycerol
\ Signal transduction
\ Antioxidant
