超临界流体色谱纯化青蒿素的研究

The Study on Purification of Artemisinin by Supercritical Fluid Chromatography

将超临界流体色谱技术应用于青蒿萃取液中青蒿素的提纯。采用ZorBaxSB-C18色谱柱(9.4mm×250mmI.D.,5μm),在改性剂含量约0%～10%,CO2流速6～30g·min·1,压力10～16MPa,温度313.15～338.15K范围内考察了青蒿素的纯化效果,确定了较适宜的色谱提纯条件为:CO2流速22g·min·1,柱温313.15K,柱压11MPa;此条件下提纯青蒿提取液,得到的产品纯度为74.83%(wt)。研究了提纯过程中的保留值规律,将青蒿素的容量因子与密度和温度的关系进行了关联,计算值和实验值的平均相对偏差为6.002%,二者吻合较好。

The technique of supercritical fluid chromatography （SFC） was introduced into the purification of artemisinin from the Artemisia annu L.extraction solution.The artemisinin was purified by SFC with a C18 column （9.4 mm×250 mm I.D.,5μm） under following conditions：the concentration of the modifier is 0%～10%,CO2 velocity is in the range of 6～30 g？min？1,column pressure is 10～16 MPa and temperature is 313.15～338.15 K.The suitable chromatographic purification conditions were determined as follows：CO2 velocity of 22 g？min？1,column temperature and pressure of 313.15 K and 11 MPa,respectively.Under above conditions,the crystal product with 74.83% artemisinin could be obtained from the Artemisia annu L.extraction solution by SFC.The law of the retention in SFC purification and the preparation process of artemisinin product were studied,and the capacity factor of artemisinin was correlated with the CO2 density and temperature.The calculated and experimental data are in good agreement and with an average relative deviation of 6.002%.