发酵液中1,3-二羟基丙酮的分离提取研究

Extraction of 1,3-Dihydroxyacetone from the Fermentation Broth

对氧化葡萄糖酸杆菌CGMCC1.23突变株GM51发酵得到的1,3-二羟基丙酮(DHA)发酵液,首先采用300mg?L?1天然高分子絮凝剂壳聚糖和3%(w/v)活性炭对DHA发酵液进行预处理,然后浓缩发酵液至含水量为10%～20%范围内,加入体积比为3:1的乙醇醇沉脱盐和蛋白杂质后,蒸发发酵液,于5℃大于90%乙醇水中结晶得到DHA产品。结果表明整个工艺过程中DHA产品的收率为72%,高效液相色谱检测DHA晶体纯度99%。本研究结果对发酵液中DHA大规模分离提取应用提供了可靠的基础。

The technique of alcohol precipitation-evaporative crystallization used for the extraction of 1,3-dihydroxyacetone （DHA） from the fermentation broth was studied.The DHA fermentation broth was produced by the fermentation of the mutant GM51 of Glucobnobacter oxydans CGMCC 1.23.The DHA fermentation broth was pretreated firstly by adding 300 mg？L？1 natural macromolecular flocculant—chitosan to remove the cells and protein,and then by adding 3%（w/v） absorbent charcoal to decolour the broth.The pretreated fermentation broth was then condensed to be having water content of 10%～20% by vacuum evaporation.The alcohol with the volume ratio of 3：1 to concentrate was added to remove the salt ions and protein in the concentrate by precipitation.The liquid phase obtained after precipitation was evaporated under vacuum,and the DHA was crystallized at 5℃ from alcohol solution with alcohol concentration of 90% or more.Through above processes,the yield of DHA is 72% and the purity of DHA determined by HPLC is up to 99%.The experimental results indicate that using this technology could obtain high DHA yield and high purity.The study offers a reliable foundation for the extraction of DHA from fermentation broth in large-scale.