抗感染重组合异种骨生物活性的研究

Bioactivity of Antibiotic Supplemented Reconsitituted Bone Xenograft

目的：探索开放性骨折骨缺损一期植骨的新途径。材料和方法：以重组合异种骨为载体，采用庆大霉素溶液浸泡和庆大霉素———明胶包裹２种方法制成复合植骨材料，以小鼠肌袋法分别观察此复合植骨材料的成骨活性和体内药物释放特性。结果：２种复合材料的成骨活性均与单纯重组合异种骨无明显差别（Ｐ＞００５），均较单纯牛松质骨载体成骨活性好（Ｐ＜００１）。２种复合材料均于３ｄ内骨粒及其周围软组织的庆大霉素浓度高于金黄色葡萄球菌的最小抑菌浓度，１２ｈ时在软组织中分别产生１６５６μｇ／ｍｌ和３０４５μｇ／ｍｌ的高浓度。结论：抗感染重组合异种骨有较好成骨活性和一定的抗感染能力，具有较好的临床应用前景。

Objective:This study was designed to explore new ways of bone grafting during primary care in treating contaminated bony defects.Methods:Two approaches were employed:RBX impregnated with gentamicin solution;RBX covered with mixture of collagen and gentamicin.Results:Bioassay in mouse thigh pouch showed that there was no significant difference in osteo inductivity between antibiotic supplemented RBX and RBX alone in same dose of BMP( P >0 05),but demonstrated stronger osteoinductive activity compared to bovine cancellous carrier ( P <0 01).While 169 6μg/ml was detected in the eluate around RBX impregnated with gentimicin bone powder at 12h,and within 4 days,the concentration was above the MIC of S.aureus;304 5μg/ml was detected in the eluate around RBX covered with mixture of collagen and gentamicin bone power,and within 3 days,the concentration was above the MIC of S aurers.Conclusion:Antibiotic supplemented RBX has high osteoinductive activity and bactericidal activity,thus predict its potential use in treating contaminated bony defeats.