三疣梭子蟹溶藻弧菌疾病模型的构建

Establishment of a Vibrio alginolyticus Disease Model for the Crab, Portunus trituberculatus

采用溶藻弧菌(107 CFU.mL-1)对三疣梭子蟹(Portunus trituberculatus)进行人工感染试验,于感染后0、24、48和72h分别观察蟹体的行动、体表及内部器官组织的病理变化,测定血细胞密度(THCs)及血淋巴、肌肉、肝胰腺3种主要组织中的碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、过氧化物酶(POD)活性和总抗氧化能力(T-AOC)水平.结果表明:三疣梭子蟹感染溶藻弧菌24h后其行动、体表及内部器官组织等病理症状逐渐明显,肝胰腺、血淋巴、肌肉等3种主要组织中的AKP、ACP、POD活性和T-AOC水平及THCs变化显著,可作为疾病模型构建的指标.由于药物防治主要针对早期发病群体,因此,疾病模型构建的时间选择病原菌感染后24h.采用上述方法构建的三疣梭子蟹溶藻弧菌疾病模型在相同条件下可重复性较强,并便于观测感染前后蟹体的变化情况.

Crabs,Portunus trituberculatus,were infected artificially with Vibrio alginolyticus with a concentration of 107 CFU·mL-1.The external symptoms and histopathological changes of infected crab were observed and the physio-biochemical indexes were determined at 0,24,48 and 72 h post-injection.The results indicated that the pathological changes of the infected crab were obvious gradually at 24 h post-injection and THCs、T-AOC、AKP、ACP and POD had significant changes,so the indexes can be used to determine whether the establishment of disease model was successful in this paper.The main objection of drug prevention was early diseased population,the time of establishment of disease model was at 24 h post-injection.The Vibrio alginolyticus disease model for Portunus trituberculatus which was established by this method was reproducible highly and convenient for observing changes of crabs.