肺癌患者支气管灌洗液的P53基因突变研究

ANALYSIS OF ACTION OF P53 GENE MUT ATIONS IN BRONCHIAL LAVAGE FLUID FROM 23 PATIENTS WITH LUNG CANCER

为了探讨抑癌基因P53与肺癌的关系．本组实验应用PCR－SSCP法对23例肺癌患者支气管灌洗液进行P53基因突变检测．结果发现23例肺癌支气管灌洗液的P53基因突变率为52．17％，进一步分析发现，P53基因突变与肺癌病理类型、临床分期以及局部淋巴结是否转移无关（P＞0．05）。提示：P53基因突变在肺癌发展中可能发生较早．检测肺癌支气管灌洗液P53基因突变有助于肺癌的早期诊断。

To studg the relation between P53 mutations and lung cancer. Aberrations of the P53 gene (exon5-8) were analyzed in 23 bronchial lavage fluid samples from 23 patient with lung cancer by he PCR-SSCP method- the results showed that 52. 17% of bronchial lavage nuid had P53 gene mutations, did not correlate with pathological types clinical staying and lymph node metastasis.these results indicated that P53 gene mutationsmay be an early event in the progression of lungcancer and the dedection of P53 mutations inbronchial lavage fluid attribute to early diagnosis of lung cancer.