内蒙古地区蒙汉族人类白细胞抗原-DQB1基因多态性与支气管哮喘相关性研究

Association between human leukocyte antigen DQB1 gene polymorphisms and bronchial asthma among Mongolia and Han nationalities

目的 探讨蒙汉民族人群人类白细胞抗原（HLA）-DQB1基因多态性与支气管哮喘的相关性.方法 2007年3月至2009年3月采用序列特异性引物聚合酶链反应（PCR-SSP）法对汉族哮喘患者50例、蒙古族哮喘患者68例进行HLA-DQB1等位基因频率的检测,并分别与50例健康汉族和54例健康蒙古族进行比较.结果 汉族支气管哮喘HLA-DQB1 0602基因频率高于汉族健康对照组（OR=6.163,P＜0.01）;蒙古族支气管哮喘者HLA-DQB1 0603/0608基因频率低于健康蒙古族对照组（OR=0.199,P＜0.05）;蒙古族支气管哮喘HLA-DQB1 0301/4基因频率高于健康蒙古族对照组（OR=2.074,P＜0.05）;蒙古族支气管哮喘HLA-DQB1 DQB1 0301/4基因频率高于汉族哮喘组（OR=2.482,P=0.05）;健康蒙古族组HLA-DQB1 0602基因频率高于健康汉族对照组（OR=3.341,P＜0.05）;健康蒙古族组HLA-DQB1 0402基因频率低于健康汉族对照组（OR=0.209,P＜0.05）.结论 HLA-DQB1 0603/0608基因可能是蒙古族哮喘患者的保护基因,HLA-DQB1 0301/4基因可能是蒙古族哮喘患者的易感基因,HLA-DQB1 0602基因可能是汉族支气管哮喘患者的易感基因.

Objective To investigate association between human leukocyte antigen DQB1 （HLADQB1 ） gene polymorphisms and bronchial asthma among Mongolian and Han nationalities. Methods Sequence-specific primer polymerase chain reaction （PCR-SSP） was used to detect frequencies of HLA DQB1 genotypes and alleles in 50 cases of Han and 68 Mongolian asthmatic patients, and 50 Han and 54 Mongolian healthy controls, respectively. Difference in gene frequencies between the two nationalities was estimated by odds ratio （OR） and chi-square test. Results Frequency of the HLA-DQB1 0602 allele was significantly higher in Han patients with bronchial asthma than that in healthy Han nationality （OR = 6.163,P ＜0.01 ）. Frequency of the HLA-DQB1 0603/0608 allele decreased in Mongolian asthmatic patients, as compared to that in healthy Mongolians （ OR = 0.199, P ＜ 0.05 ）. Frequency of the HLA-DQB1 0301/4 allele was significantly higher in Mongolian asthmatic patients as compared to that in healthy Mongolians （ OR =2.074,P ＜0.05）. Frequency of the HLA-DQB1 0301/4 allele was significantly higher in Mongolian than that in Han asthmatic patients （ OR = 2.482 ,P =0.05）. Frequency of the HLA- DQB1 0602 allele was significantly higher in healthy Mongolians than that in healthy Han nationality （ OR = 3.341, P ＜ 0.05 ）, in contrast, frequency of the HLA-DQB1 0402 allele was significantly lower in healthy Mongolians than that in healthy Han nationality （ OR = 0.209, P ＜ 0.05 ）. Conclusions The HLA-DQB1 0603/0608 allele is possibly a protective gene and the HLA-DQB1 0301/4 allele a susceptible gene for bronchial asthma in Mongolians, and the HLA-DQB1 0602 allele is possibly a susceptible gene for bronchial asthma in Han nationlity.