摘要
为观测糖尿病大鼠脊髓水平（背角浅层和腹角运动神经元）降钙素基因相关肽（ＣＧＲＰ）的含量及电针的治疗效应，采用免疫组织化学方法结合图像分析。结果：①造模三个月的糖尿病（ＤＭ）组脊髓背角浅层ＣＧＲＰ的相对含量与正常（ＮＤ）组相比显著降低（Ｐ＜０．００１）；经电针治疗后ＣＧＲＰ含量与 ＮＤ组比较虽也下降（Ｐ＜０．０１或 ０．００１），但与 ＤＭ组相比明显减轻，有极显著差异（Ｐ＜０．００１）。造模六个月的ＤＭ组及电针治疗组的情形与造模三个月时相似。②造模三个月的ＤＭ组与ＮＤ组相比，脊髓腹角ＣＧＲＰ阳性运动神经元所占百分比显著减少，强度明显减弱（Ｐ均＜０．００１）。经电针治疗后 ＣＧＲＰ阳性运动神经元所占百分比与 ＮＤ组相似（Ｐ＞０． ０５），但强度稍弱（ Ｐ＜ ０． ０５）。结论：糖尿病大鼠脊髓背角浅层及腹角运动神经元 ＣＧＲＰ含量均下降，电针治疗对这些变化具有一定的防治作用。
Immunohistochemical methods combined with computer image pattern analysis revealed that, (1 ) the relative content of CGRP in the superficial layer of spinal dorsal horn was 70. 35 ± 1. 29 in age matched (ND) group and 45. 08 ± 1. 35 in diabetic mellitus (DM) group modellized for 3 months, dropped significantly (P < 0. 001), which of electroacupuncture (EA) group was 58. 48 ± 1. 11, elevated significantly compared with DM group (P < 0. 001 ). The content of CGRP of DM group modellized for 6 months was similiar with that for 3 months; (2) the percentage and the intensity of CGRPLI positive neurons in spinal ventral horn of DM group for 3 months dropped significantly respectively compared with that of ND group (P < 0. 001 ), but the percentage of CGRP-LI positive neurons in EA group was similiar with (P >0. 05 ), and the staining intensity in EA group was weaker than (P < 0. 05 ) that of age matched group. These results indicate that CGRP contents in the superficial layer of spinal dorsal horn and ventral horn decrease in DM group, and EA can prevent it effectively.
出处
《针刺研究》
CAS
CSCD
1999年第2期126-130,共5页
Acupuncture Research
基金
国家自然科学基金!(No.39770926)
