电针抗大鼠急性炎症性内脏痛的肠神经机制

Study on the Enteric Nervous Mechanism of Electroacupuncture Anti-acute Inflammatory Visceral Pain of Rat

实验用雄性 ＳＤ大白鼠 ６４只，随机分为 ４组，①正常对照组；②电针组（ＥＡ组），取双侧“足三里”、“伏兔”穴；③福尔马林诱发的急性炎症性内脏痛组（ＶＰ组）；④电针＋腹痛组（ＥＡ＋ ＶＰ组）。观察内脏痛行为变化，记录疼痛积分，用组织化学方法观察大鼠结肠和回盲部一氧化氮合酶（ＮＯＳ）阳性神经元及乙酰胆碱酯酶（ＡＣｈＥ）阳性神经元的密度变化。结果：ＥＡ＋ ＶＰ组疼痛积分明显低于ＶＰ组；ＥＡ组结肠和国盲部ＮＯＳ、ＡＣｈＥ阳性神经元胞体和纤维密度明显低于正常对照组，而 ＶＰ组结肠和国盲部 ＮＯＳ、ＡＣｈＥ阳性神经元胞体和纤维密度明显高于正常对照组， ＥＡ＋ ＶＰ组明显低于 ＶＰ组。结论：肠道氮能神经与胆碱能神经参与了内脏痛与针刺镇痛。

In order to investigate whether enteric nervous system is involved in electroacupuncture analgesia, 64 Sprague-Dawley were randomly divided into four groups, control group, electroacupuncture (EA) group given electroacupuncture at bilateral 'Futu' and 'Zusanli' points, acute inflammatory visceral pain(VP) induced by injection of formalin into the colon wall and EA + VP group. The changes of density of nitric oxide synthase(NOS)-, acetylcholinesterase (AChE ) - positive neuronal celf body and fiber in rat colon and ilioceacal valve were observed by histochemical method. Results indicate that formalin induced evident visceral pain response, the pain score in EA+ VP group was significantly smaller than that in VP group (P < 0. 01 ). The densities of NOS-, AChE-positive neuronal cell body and fiber of colon and ilioceacal valve in EA group were significantly smaller than those in control group(P < 0. 01, P < 0. 05 ), on the contrary, the densities of NOS-, AChE-positive neuron and fiber in the colon and ilioceacal valve in VP group were significantly greater than those in control group(P < 0. 01, P < 0. 05 ) , and which in VP + EA group were smaller than those in VP group(P < 0. 01, P < 0. 05 ). The results suggest that nitrergic neuron and cholinergic neuron of the enteric nervous system are involved in the electroacupuncture anti-acute inflammatory visceral pain.