摘要
采用四甲基偶氮唑蓝比色法(MTT法),考察了两亲性α-四(对-羧基苯氧基)酞菁锌(Ⅱ)光敏剂对Bel-7402人体肝癌细胞的离体光动力学行为,研究了浓度和光照时间对光灭活作用的影响.结果发现:在适宜的浓度下,酞菁锌光敏剂表现出了良好的抑制作用,且此抑制过程遵循一级反应的动力学规律.另外,酞菁在光催化下产生的单重态氧能损坏癌变的细胞膜,导致靶对细胞的死亡.
In vitro photodynamic activities of amphiphilic α-tetra-（p-carboxyphenoxy） phthalocyanine zinc（ Ⅱ ） complex toward human Bel-7402 liver cancer cell were investigated by MTT method. The photoinactivation was influenced by concentration and light irradiation time was studied. This zinc phthalocyanine as one of photosensitizers exhibits good inhibition effects under appropriate concentration, and the inhibition process follows first order reaction rule approximately. In addition, singlet oxygen species generated by phtbalocyanine under illumination could wreck the cancer cell membrane, resulting in death of target cell.
出处
《影像科学与光化学》
CAS
CSCD
北大核心
2010年第5期362-367,共6页
Imaging Science and Photochemistry
基金
黑龙江省自然科学基金重点项目(D200822)
