富硒猴头菌中含硒蛋白提取工艺研究

Study on Extracting Technology of Seleium-containing Protein in Se-enrichment Hericium

以富硒猴头为原料,采用蒸馏水、Tris-HCl、PBS、NaOH溶液、NaCl、乙醇对富硒猴头中硒蛋白的提取分离工艺进行了初步研究,结果表明,NaOH溶液的蛋白提取量和硒提取率最优。进而以NaOH溶液作为浸提液,对富硒猴头中可溶性硒蛋白的提取工艺进行了系统的研究,通过单因素试验确定的富硒猴头中硒蛋白的提取工艺为：提取温度60℃,料液比1∶20,碱液浓度0.100 moL/L,提取时间10 h,提取3次,等电点沉淀蛋白溶液pH值调节至3~4。在单因素试验基础上,进行了4因素3水平的正交试验,得出了提取的最佳条件为：提取温度70℃,料液比1∶20,NaOH溶液浓度0.07 moL/L,提取时间8 h,在此条件下硒蛋白质的提取率为67.47%。

The experiment was conducted on the method of Se-containing protein extraction process from Se-enriched Hericium in different solvent with distilled water,tris-HCl,PBS,NaOH solution,sodium chloride and ethanol.The results showed that soluble selenium-contain protein in Hericium extraction technology research base on the NaOH solvent showed a better extraction ratio compared with others.The key factor of extraction was confirmed by single-factor experiment：temperature 60 ℃,the ration of raw material to water 1 ∶20,base solvent concentration 0.100 moL/L,extraction time was 10 hrs,extraction times was 3,pH value of solution to precipitate protein solution by isoelectric ajusted from 3 to 4.An orthogonal experiment with 4 factors and 3 levels revealed the best condition of extraction as fellows：temperature 70 ℃,the ration of raw material to water 1∶20,base solvent concentration 0.07 moL/L,extraction time 8 hrs,under above conditions the Se-containing protein extraction rate was 67.47%.