凝胶色谱宽分散样品定标法及其应用

CALIBRATION METHOD AND APPLICATION OF WIDE DISPERION SAMPLE IN GPC

按照波兰学者Ｓｚｅｗｃｚｙｋ的思路，和凝胶渗透色谱定标曲线的线性部分方程ＬｏｇＭ＝ａ－ｂＶ，推导并建立了一种适用于凝胶色谱宽分散样品的实用定标法。采用的是以数均分子量为基础的迭代变量定标法，通过计算机确定ａ、ｂ值，结果表明：该方法的结果具有和Ｓｚｅｗｃｚｙｋ结果相同的可计算性。并将该方法应用于聚异丁烯和聚醚的分子量及其分布测定，取得准确、满意的结果。

According to the idea of Szewczyk (scholar from Poland) and,the linear equation of calibrationcurve in GPC (LogM = a - bV), infered and set up a kind of practical calibration method that is suitable for thewide dispersion sample of GPC which take calibration method of iteration variable based on average molecularweight. The values of a and b were calculated through BASIC language. The result showed that this method hasthe same calculability as Szewczyk's. Applied this method to determinate Mw and Mw contribution of poly-isobutylene and polyether, a satisfied effect was obtained.