感冒解毒灵颗粒中橙皮苷和牛蒡苷的HPLC法测定

Determination of Hesperidin and Arctiin in Ganmao Jieduling Granules by HPLC

建立了HPLC法同时测定感冒解毒灵颗粒中的橙皮苷和牛蒡苷。采用C18色谱柱,以1%乙酸-甲醇(52︰48)为流动相,检测波长280nm。橙皮苷和牛蒡苷在7.5～150和5～100μg/ml浓度范围内线性关系良好,平均回收率为99.2%和99.6%,RSD为0.64%和0.79%。

An HPLC method was established for the simultaneous determination of hesperidin and arctiin in Ganmao Jieduling granules. A C18 column was used with the mobile phase of 1% acetic acid-methanol（52︰48） at the detection wavelength of 280 nm. The calibration curves of hesperidin and arctiin were linear in the concentration ranges of 7.5-150 and 5-100 μg/ml. The average recoveries were 99.2% and 99.6%,with RSDs of 0.64% and 0.79%.