基因型及胚发育对百合远缘杂交胚拯救的影响

The effects of genotype and embryo development on embryo rescue for distant hybrids of lilies

以亚洲百合、铁炮百合、东方百合及喇叭百合远缘杂交子代为材料,进行了不同基因型、不同胚状态、不同胚龄的胚拯救试验。结果表明：不同基因型间胚囊（胚）获得率及萌发率均存在较大差异;成熟胚萌发率在85%以上,成熟胚囊萌发率在10%~59%,幼嫩胚囊萌发率很低,大多在20%以下;成熟胚囊、幼嫩胚囊、成熟胚组培苗生长时非正常苗出现情况亦各不相同;所有基因型最适宜的胚拯救胚龄为39~90天,不同基因型随着胚龄的不同其胚囊（胚）获得率呈现不同的变化规律。

Taking distant hybrids of Lilium Asian,L.Longiflorum,L.Oriental and L.Trumpet as study materials,the embryo rescue experiments were conducted in different genotypes,embryo states and embryo ages.The results showed that： the obtaining rates of embryo-sacs and germination rate of embryos in different genotypes were obviously different.The germination rates of mature embryos were up to 85%.The germination rates of mature embryo-sacs were between 40%~60%.And germination rates of young embryo-sacs were low,most of them were below 20%.Among in vitro plantlets,the abnormal plantlets from mature embryos,mature embryo-sacs,and young embryo-sacs were also different.The optimal embryo age for embryo rescue of all genotypes was 35~90 days.Due to different genotypes,the obtaining rates of embryo-sacs or embryos had different changing patterns in different embryo ages.