摘要
以亚洲百合、铁炮百合、东方百合及喇叭百合远缘杂交子代为材料,进行了不同基因型、不同胚状态、不同胚龄的胚拯救试验。结果表明:不同基因型间胚囊(胚)获得率及萌发率均存在较大差异;成熟胚萌发率在85%以上,成熟胚囊萌发率在10%~59%,幼嫩胚囊萌发率很低,大多在20%以下;成熟胚囊、幼嫩胚囊、成熟胚组培苗生长时非正常苗出现情况亦各不相同;所有基因型最适宜的胚拯救胚龄为39~90天,不同基因型随着胚龄的不同其胚囊(胚)获得率呈现不同的变化规律。
Taking distant hybrids of Lilium Asian,L.Longiflorum,L.Oriental and L.Trumpet as study materials,the embryo rescue experiments were conducted in different genotypes,embryo states and embryo ages.The results showed that: the obtaining rates of embryo-sacs and germination rate of embryos in different genotypes were obviously different.The germination rates of mature embryos were up to 85%.The germination rates of mature embryo-sacs were between 40%~60%.And germination rates of young embryo-sacs were low,most of them were below 20%.Among in vitro plantlets,the abnormal plantlets from mature embryos,mature embryo-sacs,and young embryo-sacs were also different.The optimal embryo age for embryo rescue of all genotypes was 35~90 days.Due to different genotypes,the obtaining rates of embryo-sacs or embryos had different changing patterns in different embryo ages.
出处
《林业科技开发》
2010年第5期18-21,共4页
China Forestry Science and Technology
基金
河北省优秀专家出国培训项目(编号:冀人字[2003]98号)
关键词
百合
远缘杂交
胚拯救
基因型
胚发育
Lilium
distant hybrids
embryo rescue
genotype
embryo development