丙酮酸乙酯对急性肝损伤大鼠血清ALT、TNF-α和HMGB1水平的影响

Effects of Ethyl Pyruvate on the Expression of ALT、TNF-α and HMGB1 in Acute Liver Injury Rats

目的:观察丙酮酸乙酯(EP)对急性肝损伤(ALI)大鼠血清ALT、TNF-α和HMGB1水平的影响,探讨EP对ALI的保护作用。方法:雄性Wistar大鼠30只,随机分为三组,每组10只。ALI组:用D-GalN/LPS复制大鼠ALI模型;EP干预组:于D-GalN/LPS注射24 h后,EP 40 mg/kg,2 mL生理盐水稀释腹腔注射;对照组:等量生理盐水腹腔注射。各组动物分别于处理后48 h腹主动脉采血,全自动生化分析仪检测血清ALT含量,ELISA方法检测血清TNF-α含量,Western blot方法检测血清HMGB1含量。结果:EP干预组血清ALT和TNF-α含量虽高于正常对照组,但显著低于ALI组(P<0.01);EP干预组HMGB1含量显著低于对照组和ALI组(P<0.01)。结论:EP可以抑制ALI大鼠血清ALT、TNF-α和HMGB1水平,减轻由炎症因子引起的ALI。

Objective： To observe the protective role of ethyl pyruvte in acute liver injury, by testing the level of serum ALT. TNF-α and HMGBI in acute liver injury rat.Methods：Thirty BW 190 g- 250 g male wistar rats were randomly divided into three groups： acute liver injury group,use D- GalN/LPS copy ALl model rats; EP interferent group,use D- GalN/LPS copy ALl model rats,after 24 h, interference with EP; and the control group.48 h after each group be management with, the parameters of serum ALT,TNF - a and HMGB 1 were measured respectively. TNF - a was observed by ELISA, and the expression of HMGB 1 was detected by Western blot. Results ： The levels of ALT and TNF - α in the EP group were much higher than that in the control group, but far lower than that in the acute liver injury group（ P 〈 0.01） .The levels of HMGBI in the EP group were much lower than that in the control group and acute liver injury group （ P 〈 0.01 ）. Conclusion：Ethyl pyruvate could inhibit the release of ALT,TNF - α and HMGB1, and play a protective role in Acute Liver Injury.