摘要
该文初步研究了荚膜黄芪苯丙氨酸解氨酶(PAL)的基本特性。结果表明:PAL的最适反应时间为30min,PAL在Tris-HCl缓冲液中适宜的pH值为8.0,最适反应温度为37℃,PAL酶浓度过高或过低不利于酶的活力,PAL最适底物浓度为2mmol/L。
The basic characteristics of phenylalanine ammoma-lyase （PAL） of Astragalus membranaceus was studied. The results showed that the optimum reaction time was 30 min, the optimum pH in Tris-HCl buffer was 8.0, the optimum reaction temperature was 37 ℃, higher or lower concentration of PAL was not approprite for the PAL activity, and the optimum substrate concentration of PAL was 2 mmol/L.
出处
《辽宁林业科技》
2010年第5期1-2,35,共3页
Liaoning Forestry Science and Technology
基金
国家自然科学基金资助项目(30860036)
关键词
膜荚黄芪
苯丙氨酸解氨酶
特性
Astraga/us membranaceus
phenylalanine ammonia-lyase
characteristics
