旋转式荧光定量PCR在临床应用中的价值

Application of Rotary Fluorescent Quantitative PCR in Clinical Detection of M.tuberculosis

目的:探讨旋转式荧光定量PCR仪在检测临床标本中的应用价值。方法:通过旋转荧光定量-PCR技术鉴定1132株临床分离株,进行结核分枝杆菌的菌种鉴定和定量分析,同时进行抗酸染色和培养法。结果:总阳性检出率为48.64%,其中菌阳检出率为96.56%(253/262),阴性符合率为77.85%(369/474),菌种鉴定特异性实验符合率为100%。结论:旋转氏荧光定量PCR仪改进后更加快速、敏感,与其配套使用的试剂特异性强,是在检测结核分枝杆菌复合群的同时,又能进行非结核分枝杆菌鉴定辅助诊断的好方法。

Objective To discuss the application of rotary fluorescent quantitative PCR instrument in clinical detection of M. tuberculosis. Methods 1 132 clinical isolated strains were tested by rotary fluorescent quantitative PCR（FQ-PCR） for strain identification and quantitative analysis and specimens were examined microscopically with Ziehl -Neelsen staining and cultured at the same time. Results Total positive detection rate was 48.6% and positive detection rate 96.56%（253/262） in positive acid fast stain with negative coincidence 77.85% （369/474）. Coincidence of strain identification was 100% . Conclusion Using rotary FQ-PCR instrument with supporting reagent can effectively identify Mycobacterium tuberculosis complex and diagnose tuberculosis.