德国小蠊的抗药性及多功能氧化酶在抗性中的作用

The insecticides resistance of German cockroach Blattella germanica L. and the role of mixed function Oxidase in insecticides resistance.

通过敏感性测定、增效作用试验和酶活力测定，分析了德国小蠊对拟除虫菊酯类药剂的抗药性 以及多功能氧化酶在抗药性中的作用，结果表明采自杭州的德国小蠊品系对二氯苯醚菊酯、溴氰菊酯和 氯氰菊酯的抗性系数分别是敏感品系的６７．１倍， １８．１倍和１１．８倍，利用多功能氧化酶的抑制剂── 氧化胡椒基丁醚作为增效剂测定抗性品系对二氯苯醚菊酯的增效作用，增效３．６９倍，离体酶活力测定 的结果表明，抗性品系中多功能氧化酶活力明显高于敏感品系，由此认为，德国小蠊的杭州品系已对拟 除虫菊酯药剂产生了抗药性，多功能氧化酶活力增强是导致德国小蠊抗药性产生和发展的重要原因。

The sensitivity of a field strain (HZ) of the German cockroach , Blattella germanica L. to per- methrin, deltamethrin and cypermethrin was tested using a topical application method to estimate LD50. The results indicate a high level of resistance in this strain to pyrethroids, especially to permethrin. The LD50 resistance ratios (RR) for permethrin , deltamethrin and cypermethrin were 67.1 , 18. 1and 11. 8 , re- spectively. Studies were conducted (in vivo) on the HZ strain to determine the synergistic effect on perme- thrin by metabolic inhibitor piperonyl butoxide(PBO). The synergistic effect on the Ld50 RR for PBO was 3. 69 times, which indicated that the mechanism for pyrethroid resistance in the HZ strain was associated with mixed function oxidases (MFOs).Biochemical analysis showed that MFOs activities in the resistant (field) strains were higher than those in the susceptible strain. These data indicate that the difference in the activity of MFOs are important factors in the development of insecticide resistance in the field strain of German cockroach evaluated.