胃痞消对脾虚型慢性萎缩性胃炎癌前病变大鼠胃黏膜上皮细胞增殖周期分布及凋亡基因表达的影响

Effect of Weipixiao on Cell Generation Cycle Distribution and Apoptosis-related Gene Expression in Gastric Mucosal Epithelial Cells of Gastric Precancerous Lesion Rats with Spleen-deficiency Chronic Atrophic Gastritis

【目的】观察健脾化瘀解毒中药胃痞消(由黄芪、太子参、白术、丹参、白花蛇舌草等组成)对脾虚型慢性萎缩性胃炎(CAG)癌前病变大鼠胃黏膜上皮细胞的增殖周期分布及凋亡基因表达的影响。【方法】选用SD大鼠,随机分为6组,即正常对照组,模型组,胃痞消高、中、低剂量组(剂量分别为15、7.5、3.75g·kg-1·d-1),维酶素组(剂量为0.27g·kg-1·d-1)。除正常对照组外,其他组均采用N-甲基-N'-硝基-N-亚硝基胍(MNNG)液自由饮用结合饥饱失常加耗气泻下法复制脾虚型CAG胃癌前病变模型,观察胃痞消各剂量组连续治疗4周后对大鼠胃黏膜上皮细胞增殖周期分布、凋亡率、凋亡基因p53、Bcl-2和Bax表达的影响。【结果】胃痞消各剂量组可显著增加胃黏膜上皮细胞凋亡率,增加G0/G1期、减少S期细胞分布,促进p53表达,抑制Bcl-2表达(均P<0.01),胃痞消高剂量组可显著促进Bax表达(P<0.05)。【结论】胃痞消可抑制CAG癌前病变大鼠胃黏膜上皮细胞的增殖,促进细胞凋亡,其机制可能与其能促进p53和Bax表达,抑制Bcl-2表达有关。

Objective To observe the effect of Weipixiao Pseudostellariae, Rhizoma Atractylodis Macrocephalae, Radix （mainly composed of Radix Astragali , Radix Salviae Miltiorrhizae , Herba Hedyotis Diffusae , etc. ） on cell generation cycle distribution and apoptosis-related gene expression in gastric mucosal epithelial ceils of gastric precancerous lesion rats with spleen-deficiency chronic atrophic gastritis （CAG）. Methods SD rats were randomized into 6 groups ： normal control group, model group, Vitacoenzyme Tablets group （0. 27 g · kg^- 1 · d ^-1 ） , and high-, middle- and low-dosage Weipixiao groups （ 15.00, 7.50 and 5.75 g · kg^- 1 · d ^-1, respectively）. Except for the normal control group, the rats in other groups received spontaneous intake of N-methyl-N′-nitro-N- nitrosoguanidine （MNNG） solution combined with irregular diet and purgative herbs for 12 weeks to induce spleen- deficiency CAG gastric precancerous lesion . After treatment for 4 weeks, cell generation cycle distribution, apoptotic rate, and apoptosis-related genes （p53, Bcl-2 and Bax） expression in gastric mucosal epithelial cells were observed. Results Apoptotic rate and the number of Go/GI phase cells were increased, the number of S phase ceils was decreased, p53 expression was enhanced, and Bcl-2 expression was inhibited in Weipixiao groups （P 〈 0.01 ）. High-dosage Weipixiao markedly promoted Bax expression （P 〈 0.05 ）. Conclusion Weipixiao can inhibit the proliferation of gastric mucosal epithelial cells of gastric precancerous lesion rats with spleen-deficiency CAG, and promote apoptosis. Its therapeutic mechanism is probably related with the promotion of p53 and Bax expression, and with the inhibition of Bcl-2 expression.