细菌纤维素/透明质酸复合材料的生物合成及表征

Biosynthesis and Characterization of Bacterial Cellulose/Hyaluronic Acid Composites

在培育细菌纤维素(BC)过程中添加不同分子量的两种透明质酸(HA),分别制备出不同的细菌纤维素复合物HA/BC(Mw=3,000)和HA/BC(Mw=300,000)。采用红外光谱、扫描电子显微镜、X射线衍射和热重分析对其结构和性能进行了表征。添加HA后提高了复合物的产量;FTIR结果表明了HA与BC之间存在交联;添加HA增大了BC的热稳定性,而对BC的结晶指数影响不大,且HA/BC(3,000)的性能始终优于HA/BC(300,000);HA(3,000)增大了BC的拉伸强度,而HA(300,000)反而减小了其拉伸强度。结果表明添加小分子量的HA可制备最大热失重温度较高的HA/BC复合物。

Bacterial cellulose/hyaluronic acid composites（HA/BC） were obtained by adding HA（Mw=3,000） and HA（Mw=300,000） into the Acetobacter xylinum culture system.The structure and properties of the composites were determined by Fourier transform infrared spectroscopy（FTIR）,X-ray diffraction（XRD）,scaning electron microscopy（SEM） and thermogravimetric analysis（TGA）.The added HA could increase the productivity.And the FTIR spectra indicated the intermolecular interaction between BC and HA.The added HAs could increase the thermal stability of BC,but showed no significant influence on the crystallinity index（CI）,and HA/BC（3,000） had always-superior properties.The tensile strength of BC was increased by the addition of HA（3,000） but decreased by HA（300,000）.The results show that HA/BC have the increased max weight loss temperature with addition of HA of low-molecular-weight in the culture medium.