栽培大豆与野生大豆联会复合体的电镜研究

STUDIES ON SYNAPTONEMAL COMPLEX IN GLYCINE MAX AND GLYCINE SOJA BY EM

以改进的去污剂微铺展技术制备大豆减数分裂联会复合体标本，并对联会复合体发育的过程进行了电镜观察。结果表明：大豆ＳＣ以多点或起始方式于偶线期开始形成；随着ＳＣ的发育新的ＳＣ形成和已有ＳＣ片断的延伸并存；ＳＣ成熟于粗线期，而以破碎方式解体消失于双线期。本文列出了栽培大豆和野生大豆的ＳＣ核型，并对联会复合体侧轴加厚的数目、分布形式作了描述。

A modified detergent spreading technique was used in ultrastructural study of the development of synaptonemal complex in soybean. At zygotene stage, the SC formation inititated at multiple sites in each bivalent but the regions near telomeres were preferred with development of SC. Initiation of new SC and extension of existing SC cocured simultaneously. SC matured at pachytens. At diplotene, SC broke down and disappeard. In addition compared the nuclear type of SC between Glycine max and Glycine soja.