摘要
目的探讨不同温度、不同贮存时间对全血糖化血红蛋白(HbA1c)检测结果的影响。方法采用离子交换高效液相色谱法(HPLC)检测20例患者的全血标本,分别在不同温度(4～8℃、17～23℃、-20℃)、不同贮存时间(1、5、7、18、25、28、50d)测定HbA1c。将所测结果与新鲜全血标本所测结果进行比较。结果在4～8℃下贮存50d,全血标本HbA1c检测结果与新鲜标本所测结果比较,差异无统计学意义(P>0.05);在17～23℃下贮存7d,全血标本HbA1c检测结果较新鲜标本所测值显著下降,差异有统计学意义(P<0.05);在-20℃下贮存28d,全血标本HbA1c所测结果较新鲜标本所测值显著下降,差异有统计学意义(P<0.05)。结论不同贮存温度及不同贮存时间对全血HbA1c的检测有显著影响。
Objective To study the influence on HbA1c testing results by different storage temperature and time.Methods HbA1c of 20 whole-blood samples was tested by ion exchange High Performance Liquid Chromatography （HPLC） under different temperatures（4～8 ℃,17～23 ℃,-20 ℃） and in different time （1 d,5 d,7 d,18 d,25 d,28 d,50 d）,and the results were compared with fresh blood samples.Results After 50 days of storage under the temperature of 4～8 ℃,the result shows no significant difference compared with fresh blood samples （P0.05）.After 7 days of storage under 17～23 ℃,the result difference has statistical significance（P0.05）.After 28 days of storage under-20 ℃,the result difference has statistical significance（P0.05）.Conclusion The time and temperature of storage have significant impact on the testing result of HbA1c.
出处
《中国全科医学》
CAS
CSCD
北大核心
2010年第26期2988-2989,共2页
Chinese General Practice
