二硫化碳接触者血清铜锌超氧化物歧化酶与丙二醛水平观察

Serum Cuprozinc\|Superoxide Dismutase andMalonyldialdehyde Levels in CarbonDisulfide Exposed Workers

应用酶联免疫吸附试验（ＥＬＩＳＡ）与硫代巴比妥酸比色法对某化纤厂６７名二硫化碳（ＣＳ２）接触工人和８８名非接触者血清铜锌超氧化物歧化酶（ＣｕＺｎＳＯＤ）和丙二醛（ＭＤＡ）水平进行调查。结果显示：不同浓度ＣＳ２接触工人血清ＣｕＺｎＳＯＤ水平均显著高于对照组（Ｐ＜００００１）呈一定剂量－效应关系。以装拆等工段工人的血清ＳＯＤ水平增高为著。以接触指数（ＥＩ）分组则见，ＥＩ在６００以下时ＳＯＤ随ＥＩ的升高而增加，ＥＩ大于９００时ＳＯＤ似呈下降趋势而ＭＤＡ水平则升高。作业工人血清ＳＯＤ与ＭＤＡ测定结果提示，ＣＳ２对ＳＯＤ的影响呈双向性，当较长时间接触较高浓度ＣＳ２时机体抗氧化防御系统清除自由基能力达上限，最终将导致脂质过氧化产物堆积。血清ＳＯＤ与ＭＤＡ似有希望成为ＣＳ２接触工人的健康监护指标，有必要作进一步研究验证。

An enzyme\|linked immunosorbent assay(ELISA)was conducted on 67 workers in a viscose rayon factory to detect their serum cuprozinc superoxide dismutase(CuZnSOD)values,and a BAT was conducted to detect the serum MDA levels.Results showed that the average levels of CuZnSOD in carbon disulfide exposed workers both above and below 10 mg/m3 were higher than those of the control group( P <0\^0001),showing some dose\|effect relationship.The contents of SOD were distinctively high in those maintenance workers.SOD levels increased when the exposure index(EI)was less than 600,and tended to decrease when EI was higher than 900.Meanwhile,the serum malonyldialdehyde(MDA) levels increased.It suggested that there are some changes of antioxidation system in carbon disulfide exposed workers,Serum SOD and MDA might become objective indexes in workers health surveillance and the importance of these two indexes in clarifying the intoxication mechanism still needs to be proved.\;