人精子诱变检测中新体外代谢活化系统的研究

THE STUDY ON NEW IN VITRO METABOLIC ACTIVATION SYSTEM IN GAMETE MUTATION TEST

人精子与去透明带金黄地鼠卵受精，在受精后异合卵培养阶段，用终浓度分别为２０μｇ／ｍｌ、４０μｇ／ｍｌ、６０μｇ／ｍｌ的间接诱变剂环磷酰胺进行处理，继而制备精子染色体进行核型分析。得到环磷酰胺诱发的染色体畸变精子率在上述剂量组依次为３３％、５０％、３３％；断裂均数依次为０．５４、１．９４和０７２。与空白对照组比较，差异具有显著性（Ｐ＜０．０５，Ｐ＜００１）。本研究结果表明，用人精子／金黄地鼠异合卵为靶细胞，能够检出间接诱变剂的诱变性。异合卵内源性代谢活化体系的发现和应用在哺乳动物配子的毒理遗传研究中具有重要意义。

The zonafree golden hamster oocytes were genetrated in vitro by human spermatozoa. The hybrid zygotes were exposed to promutagen cyclophosphamide(CPA) at the concentrations of 20g/ml, 40g/ml,60g/ml respectively during the peroid of postfertilization culture. Then human sperm chromosomes were prepared and analyzed. The frequencies of the spermatozoa with structural aberrations were 33%,50%,33% and the means of chromosomal breakages were 0.54,1.94,0.72 successively in the above dosegroups. The differences of two parameters between the test groups and the controls were significant ( P<0.05,P<0.01). The results indicated that the mutagenicity of the promutagens could be evaluated in human/golden hamster hybrid zygotes as the endogenous metabolic activation system. This finding would be very beneficial for toxigenetic research in mammalian gamates.\=