应用等位基因特异性PCR和多重差别PCR检测肺癌患者CYP1A1和GSTM1的遗传多态性

IDENTIFICATION OF GENETIC POLYMORPHISM OF CYP1A1 AND GSTM1 IN LUNG CANCER PATIENTS BY USING ALLELESPECIFIC PCR AND MULTIPLEX DIFFERENTIAL PCR

本文对等位基因特异性ＰＣＲ（Ａｌｅｌｅｓｐｅｃｉｆｉｃ，ＡＳ）和多重差别（Ｍｕｌｔｉｐｌｅｘｄｉｆｅｒｅｎｔｉａｌ，ＭＤ）ＰＣＲ技术进行了优化，并用此法联合检测了１０５例江苏地区健康人群及６８例肺癌患者ＣＹＰ１Ａ１、ＧＳＴＭ１的等位基因型。结果表明：ＡＳＰＣＲ及ＭＤＰＣＲ采用的设立双参照扩增体系，可一次同时检测ＣＹＰ１Ａ１和ＧＳＴＭ１的等位基因型。在肺癌患者组中，ＣＹＰ１Ａ１的突变型Ｖａｌ／Ｖａｌ的频率１２／６８（１７．６％）约为健康组９／１０５（８．５７％）的２０５倍，而ＧＳＴＭ１纯合缺失的频率，肺癌组为３９／６８（５７３％），与健康对照组４２／１０５（４０％）相比，亦有显著增加（Ｐ＜０．０５）。

Allelespecific PCR(ASPCR) and multiplex differential PCR (MDPCR) were used to identify the genotypes of CYP1A1 and GSTM1 in 68 lung cancer patients and 105 healthy controls. The results showed that genotypes of CYP1A1 and GSTM1 could be detected simultaneously with ASPCR and MDPCR; The frequency of CYP1A1Val/Val in lung cancer patients group was 12/68(17.6%), about 2.05fold than that in healthy controls 9/105(8.57%), and the frequency of GSTM1 0/0 was 39/68(57.4%), remarkably higher compared with 42/105(40%) in healthy controls ( P <0.05)