用Ames法和致畸试验研究醋氨己酸锌的遗传毒性

STUDY ON THE GENOTOXICITY OF ZINC ACEXAMATIE BY THE AMES TEST AND TERATOGENIC TEST

用Ａｍｅｓ试验和致畸试验对醋氨己酸锌进行了致突变和致畸作用研究。结果表明醋氨己酸锌在Ａｍｅｓ试验的ＴＡ９７、ＴＡ９８、ＴＡ１００和ＴＡ１０２４个标准菌株±Ｓ９ｍｉｘ条件下，１－５０００μｇ／皿６个剂量的标准平皿掺入试验中，未诱发各菌株阳性突变反应。小鼠致畸试验３２７９ｍｇ／ｋｇ·ｂｗ、２６２３４ｍｇ／ｋｇ·ｂｗ和５２４６７ｍｇ／ｋｇ·ｂｗ剂量组，在母鼠妊娠期６－１５ｄｐ．ｏ给药，各剂量组活胎率与溶剂对照组比较，无显著差异（Ｐ＞００５）。对胎鼠外观、骨骼和内脏无致畸作用。但５２４６７ｍｇ／ｋｇ·ｂｗ组对妊娠母鼠体重、胎鼠体重和尾长有影响，显示一定的母体和胚胎毒性。

This paper reported the research results of mutagenicity and teratogenicity effect of zinc acexamatie. The result showed that zinc acexamatie with doses level (1-5000g/plate) did not induce positive mutations of strains TA\-\{97\}TA\-\{98\}TA\-\{100\} and TA\-\{102\} with or without S\-\{9mix\} in Ames test. It was divided into three groups of Zinc acexamatic received (po) dosages of 32.97mg/kg.body weight, 262.34mg/kg.body weight, 524.67mg/kg.body weight on the 6th to 15th day of gestation mouse respectively in the teratogenic test. The frequency of live fetuses of each dose group were no significant difference as compared with solvent control group. It did not cause deformity of the fetus appearances, bone and internal organs. But there were significant differences between solvent control group and 524.67mg/kg group in the growth of body weight in gestation of mouse, body weight and tail length in fetus. The result showed that zinc acexamatie possess maternal toxicity and embryotoxicity.\=