摘要
采用常规的细菌分离纯化方法从土壤和活性污泥中分离出237株菌株,通过考察菌株的絮凝能力及稳定性,从中筛选出一株絮凝剂菌,命名为HT1-2,根据其生理生化实验结果,初步鉴定该菌株为芽孢杆菌属(Bacillus)。对HT1-2的培养条件进行优化,通过单因素和正交实验得到其最优培养条件为:碳源(葡萄糖)2.0%(质量分数),氮源(氯化铵)0.2%(质量分数),菌液接种量10%(质量分数),培养温度30℃,摇床转速120r/min,发酵液初始pH=8。在此条件下培养的HT1-2对炼油废水中石油类去除率达40%以上。
237 strains were isolated from activated sludge and soil by conventional isolation and purification method.A high efficient flocculant-producing strain,which possessing perfect flocculability and stability was screened from 237 strains.The strain was designated HT1-2 and identified as Bacillus based on its physiological and biochemical characteristics.The batch treatment of oil refinery wastewater by strain HT1-2 was performed to determine the effect of culture conditions （initial pH,culture temperature,shaker speed and so on） on flocculation performance.Results showed that strain HT1-2 presented perfect flocculability and stability;the optimum culture conditions were obtained from the single factor and orthogonal experiments.Under these optimum conditions,the oil removal rate could reach more than 40%.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2010年第9期25-28,36,共5页
Environmental Pollution & Control
基金
中国石油天然气股份有限公司化工与销售分公司科学研究与技术开发项目(No.0604C18-12)
中国石油天然气集团公司应用基础研究计划课题(No.06A50302)
关键词
微生物絮凝剂
炼油废水
单因素实验
正交实验
microbial flocculant
refinery wastewater
single factor experiment
orthogonal experiment
