维药罗勒提取物对脂多糖诱导RAW264.7细胞环氧化酶-2酶活性的影响

Effect of Different Polar Extracts of Uighur Ocimum basilicum L. on COX-2 Enzyme Activity in LPS-induced RAW264.7 Cells

目的探讨维药罗勒不同提取部位在体外对COX-2酶活性的影响,进而找出该药的抗炎作用机制及有效部位群。方法 MTT法筛选维药罗勒不同提取部位的细胞给药浓度,并对脂多糖诱导的RAW264.7细胞进行不同时间的药物干预,用ELISA法检测细胞上清液中PGE2的含量。结果各部位在不同时间内均对PGE2有抑制作用,其中醋酸乙酯及正丁醇部位对PGE2有较大的抑制作用。结论维药罗勒可通过直接抑制COX-2的酶活性产生抗炎作用,醋酸乙酯及正丁醇部位是该药的有效部位群。

Objective To study the effect of Uighur Ocimum basilicum L.on COX-2 enzyme activity in LPS-induced RAW264.7 cells,and find its anti-inflammatory mechanism and effective parts.MethodsMTT method was used to the eligible concentration of different polar extracts of Uighur Ocimum basilicum L..After RAW264.7 were interfered with 2 mg·L-1 LPS for 8 h,and then different polar extracts of Uighur Ocimum basilicum L.for 30 min,the concentration of PGE2 in supernatant was analyzed by ELISA.After RAW264.7 cells were pretreated with different polar extracts of Uighur Ocimum basilicum L.for 30 min,and then 2 mg·L-1 LPS for 24 h,the concentration of PGE2 in supernatant was analyzed by ELISA.ResultsThe concentration of PGE2 was inhibited by different polar extract of Uighur Ocimum basilicum L..The inhibition ratio by acetidin part and n-butanol part were higher.ConclusionThe anti-inflammatory activity of Uighur Ocimum basilicum L.was related to inhititing the activity of COX-2 enzyme,and the effective parts are acetidin part and n-butanol part.