甲状腺转录因子-1对鸡胚尿囊膜血管生成的实验研究

Experimental Study on the Effect of TTF1 in Angiogenesis in Chick Chorioallantoic Membrane

目的研究甲状腺转录因子-1(TTF1)对鸡胚尿囊膜(CAM)血管生成的影响。方法利用CAM模型,60枚新鲜种蛋随机分为3组:PBS组,25μg/mlTTF1组,50μg/mlTTF1组,每组20只。种蛋消毒后,放入37.8℃恒温箱内孵育6d,暴露尿囊膜,将含有待测品的滤纸放在尿囊膜血管较少部位,继续孵育2d,以滤纸为中心,把膜剪下,固定,拍照并记录各组CAM血管生长及血管数目情况。结果 PBS组CAM血管及其网络清晰可见,生长良好;给药组血管数目较PBS组明显减少,血管结构模糊,颜色变浅、苍白。结论 TTF1对CAM血管生成有明显的抑制作用。

Objective To investigate the effects of compound extract from Sorbaria sorbifolia（SS）on angiogenesis in the chick chorioallantoic membrane（CAM）.MethodsSixty fresh chicken eggs were divided into three groups：PBS group,25 μg/ml TTF1 group,50 μg/ml TTF1 group,20 in each group.After disinfection,the eggs were incubated for eight days in a constant temperature box with the temperature being controlled within 37.8℃,then exposed CAM,laid the filter papers with specimen on the CAM and the eggs were incubated for another two days.The CAM,was cut,the photos were taken and the angiogenesis in the CAM was observed.ResultsThere were many CAM vessels in the PBS group and the blood vessel net could be seen clearly.The number of CAM vessels in the TTF1 groups was much less than that in the PBS group.Furthermore,the frame of the vessels was not clear,and the color was obscure.ConclusionTTF1 can obviously restrain the angiogenes in CAM.