鸡流感病毒SAα-2,3 Gal和α-2,6 Gal受体分布特点及其在流感病毒生态系统中的作用

DISTRIBUTION CHARACTERISTICS AND ROLES OF SIALIC ACID α-2,3 GAL AND α-2,6 GAL OF CHIKEN IN THE ECOSYSTEM OF INFLUENZA A VIRUS

目的:探讨鸡流感病毒受体分布特点及其在流感病毒生态系统中的位置和作用。方法:应用凝集素组织化学染色技术检测鸡呼吸道和消化道流感病毒SA受体的分布,用荧光素Alexa 488标记的禽流感病毒H9N1、人流感病毒H1N1分别与鸡呼吸道、消化道各解剖部位结合。结果:鸡呼吸道均表达SA-α2,3Gal和-α2,6Gal受体,SA-α2,6Gal受体在气管、支气管和次级支气管呈高密度分布,而SA-α2,3Gal受体在呼吸道的分布以副支气管上皮细胞为主;消化道结肠上皮细胞SA-α2,3Gal受体呈弥漫分布,但SA-α2,6Gal受体仅少量分布。同时,禽流感病毒H9N1、人流感病毒H1N1均可与呼吸道和消化道上皮细胞结合。结论:鸡同时表达SA-α2,3Gal和-α2,6Gal受体,能与禽流感病毒和人流感病毒结合,有助于病毒基因重配,可充当产生人类流感大流行潜在的中间宿主。

Objective：To study the distribution characteristics of influenza viruses receptor in chicken,and the position and role of chicken in influenza virus ecosystem.Methods：The distribution of sialic acids receptor in chicken respiratory tract and alimentary tract was investigated by using lectin histochemical staining.The epithelial cells in respiratory tract and alimentary tract of chicken were bind by Alexa 488 labelled avian influenza H9N2 virus and human influenza H1N1 virus,respectively.Results：Both SAα-2,3Gal and SAα-2,6Gal receptors were expressed in chicken respiratory tract.The SAα2,6Gal receptor was prevalent in the trachea,bronchus and second bronchus,but was infrequent in parabronchus.On the contrary,the SAα2,3Gal receptor was more common in the parabronchus and to a lesser degree in the trachea,bronchus and second bronchus.In colon of chicken,the SAα2,3Gal receptor was diffuse distribution,but SAα2,6Gal receptor was infrequent.Furthermore,Alexa 488 labelled avian influenza H9N2 virus and human influenza H1N1 virus could bind to epithelial cells in respiratory tract and alimentary tract of chicken.Conclusion：These results suggested that chicken,which carry both SAα2,3Gal and SAα2,6Gal receptors compatible with binding of avian and human influenza viruses,could provide an environment of reassortants between avian and human influenza viruses and act as an intermediate host to generate influenza virus with pandemic potential.