脱钙骨基质的制备及与软骨细胞体外复合的实验研究

EXPERIMENTAL STUDY ON PREPARING DECALCIFIED BONE MATRIX AND COMBINATING WITH CELLS IN VITRO

目的:探寻良好的软骨组织工程支架材料。方法:制备兔脱钙骨基质(decalcified bone matrix,DBM),采用液体置换法检测经脱钙2、3、4 d支架材料的孔隙率。扫描电镜观察DBM的超微结构,以及软骨细胞与DBM体外复合培养3 d的黏附情况。结果:脱钙3 d的DBM多孔结构及空隙适合软骨细胞的黏附和增殖。软骨细胞和DBM体外培养3 d黏附良好。结论:DBM是良好的软骨组织工程支架材料。

Objective：To look for the scaffold material of tissue-en gineered cartilage.Methods：The decalcified bone matrix（DBM） of rabbits was prepared.The interval porosity of the bracket material was tested by liquid substitution method after decalcification of two,three and four days.The ultra-microstructur of DBM was observed through scanning electron microscope,and the adhere of DBM and chondrocyte after cultivate three days were also studied.Results：The porous structure of DBM decalcificated for three days fitted the chondrocyte to adhere and generation.The DBM and chondrocyte adhered well in vitro.Conclusion：The DBM was a good scaffold material of tissue-engineered cartilage.