运动性骨骼肌微损伤和修复的机制研究

Research on Mechanism of the Micro-injury and Repair of the Skeletal Muscle after Eccentric Exercise

为了探讨运动性骨骼肌微损伤和修复的机制。以大鼠离心运动为运动方式,观察运动后即刻、运动后24h、运动后48h和运动后72h血清CK、血清MDA、尿液3-MH、骨骼肌组织葡萄糖-6-磷酸脱氢酶(G6PDH)和β-葡萄糖苷酸酶(GUS)等指标的变化情况。结果发现:(1)离心运动后,血清CK活性和MDA含量升高,均在运动后24h达峰值,但它们的变化没有显著的相关关系;(2)离心运动后,尿3-MH含量急剧上升并在即刻达峰值,48h以后开始恢复;(3)离心运动后即刻骨骼肌β-葡萄糖苷酸酶含量急剧上升并达峰值,G6PDH含量明显上升,在运动后24h达峰值。揭示了:一次性离心运动能引起延迟性骨骼肌微损伤,其发生的原因可能与化学因素有关。

Objective： To research on mechanism of the micro - injury and repair of the skeletal muscle after eccentric exercise. Methods ：40 male SD rats were randomly divided into five groups： no -exercise control group, post -exercise 0hour group, post - exercise 24hours group, post - exercise 48hours group, post - exercise 72hours group, which every group has 8 rats. Exercise groups rats had to interval eccentric- exercise of 120rninutes, which the speed was 16metre/mintues, grade was - 160, rest 2 minutes after exercise 5 minutes. Rats were slay respectively Serum CK,serum MDA,musele G6PDH and muscle 13 - GUS were determined. Results ： （ 1 ） Serum CK and MDA enhanced after eccentric exercise. Them reached peak in 24 hours post - exercise, serum CK gradually resumed in 48 hours and serum MDA resumed in 72 hours post - exercise ; （2） The content of urine 3 - MH sharply enhanced and reached peak in o hour, and begin to resume in 48 hours; （3） The content of skeletal β - GUS sharply enhanced and reached peak in o hour, but can not resume in 72 hours. The content of skeletal G6PDH obviously enhanced and reached peak in 24 hour, but could not resume in 72 hours. Conclusion： One acute eccentric exercise could induce delay skeletal micro - injury, and the reason was likely for the cooperation with factors of chemistry.