2(3)-叔丁基-4-羟基茴香醚对HBV转基因小鼠肝肿瘤的防护作用研究

PROTECTION AGAINST HEPATOCARCINOGENESIS BY 2(3)TERT BUTYL 4 HYDROXYANISOLE IN HBV LARGE ENVELOPE TRANSGENIC MICE

观察抗氧化剂２（３）┐叔丁基┐４┐羟基茴香醚（ＢＨＡ）对ＨＢＶ转基因小鼠肝肿瘤的防护作用。方法２６只ＨＢＶ转基因和２４只非转基因小鼠，用生化法测定肝脏醌还原酶（ＱＲ）和谷胱甘肽Ｓ┐转移酶（ＧＳＴｓ）活性、丙二醛（ＭＤＡ）含量和免疫组化法研究肝肿瘤ＰＣＮＡ表达。结果转基因小鼠ＢＨＡ组肝癌发生率０，显著低于普饲组４２％（５／１２例）。ＢＨＡ诱导肝脏ＱＲ和ＧＳＴ活性升高３～７倍，降低ＭＤＡ。ＢＨＡ组肝腺瘤ＰＣＮＡ标记指数３．４９±２．４２％，显著低于普饲组（５．４８±２．２９％）。结论ＢＨＡ长期应用有效延缓了ＨＢＶ转基因小鼠肝肿瘤的发生发展进程。

Objective:To study the protection against hepatocarcinogenesis in HBV large envelope transgenic mice by dietary antioxidant 2(3) tert butyl 4 hydroxyanisole(BHA).Methods:By determining the enzyme activities,liver MDA and PCNA immunohistochemical staining,the protective effect of BHA was observed in 26 transgenic mice.24 non transgenic mice served as control.Results:The incidence of carcinoma was 0 in BHA group,which was significantly lower than that in common diet group(42%,5/12 cases).The NAD(P)H:quinone reductase activity and glutathinone S transferases of liver cytosols were increased to 3～7 times control values in response to BHA.The mean PCNA labeling index (3.49±2.42%) in PHA group was significantly lower than that in control(5.48±2.29%).Conclusion:Early and continued addition of BHA to the diet effectively put off hepatocarcinogenesis in HBV transgenic mice.