当归对慢性支气管炎肺泡巨噬细胞上CD11c和CD14表达的影响

Effect of Angelica sinensis Injection on CD11c and CD14 Expression in Alveolar Macrophage Membrane of Chronic Bronchitis Patients Peng Ze, Zhang Zhenxiang, Xu Yongjian, et al

目的：探讨当归对慢性支气管炎（慢支）肺泡巨噬细胞（ａｌｖｅｏｌａｒｍａｃｒｏｐｈａｇｅ，ＡＭ）膜上ＣＤ１１ｃ和ＣＤ１４表达的影响。方法：慢支缓解期患者和正常对照者各１０例。经局部支气管肺泡灌洗获取ＡＭ；加当归、脂多糖（ｌｉｐｏｐｏｌｙｓａｃｈａｒｉｄｅ，ＬＰＳ）体外培养２４ｈ后，以流式细胞仪分析其ＣＤ１１ｃ和ＣＤ１４表达的百分率；测定其胞浆游离钙水平。结果：慢支者ＡＭ膜上ＣＤ１１ｃ和ＣＤ１４的表达均显著高于正常对照者（Ｐ＜００５）；ＬＰＳ可使慢支者ＡＭ膜上ＣＤ１１ｃ的表达进一步增加（Ｐ＜００５）；当归可使慢支者ＡＭ膜上ＣＤ１１ｃ的过度表达（Ｐ＜００５）。慢支者ＡＭ胞浆中基础钙水平较正常对照者明显增高（Ｐ＜００５）；ＬＰＳ促进慢支ＡＭ胞浆游离钙水平的升高（Ｐ＜００５）；当归可抑制ＬＰＳ对慢支ＡＭ胞浆游离钙水平的升高作用（Ｐ＜００５）。结论：当归通过抑制慢支患者ＡＭ胞浆游离钙水平升高下调其ＣＤ１１ｃ的表达，提示当归对于慢支缓解期气道内非特异性炎症可能具有抑制作用。

Objective: To explore the effect of Angelica sinensis injection (ASI) in CD11c and CD14 expression in alveolar macrophage (AM) membrane of chronic bronchitis patients. Methods: AM from 10 chronic bronchitis patients (remmision stage) and 10 healthy subjects was obtained by bronchoalveolar lavage. After had cultured for 24 hours with ASI or/and lipopolysaccharide (LPS), CD11c and CD14 expression and intracellular calcium ion concentration in AM were analyzed by flow cytometry. Results: CD11c and CD14 expression levels of patients' AM membrane (n=10) were (39 17±5 56)% and (35 73±8 05)% respectively, which were significantly higher than those of the control n=10, (16 29±3 78)% and (15 26±5 96)% , P<0 05. LPS could increase the elevation of CD11c expression of patients (47 25±7 00)%, P<0 05 , while ASI could reduce the increment LPS Plus ASI group (27.61±8 64)%, P<0 05 . The basic calcium level of AM cytoplasma of patients was (189.47±23.69) nmol/L (n=7), which was higher than that of healthy control (99.65±32.21) nmol/L (n=6), P<0 05. The intracellular calcium ion elevation in AM of patients could be induced by LPS, and ASI could inhibit the elevation. The calcium level in LPS group was (288.47±43.68) nmol/L, in ASI Plus LPS group, (236.68±28.60) nmol/L, P<0 05. Conclusions: ASI could reduce the CD11c expression in AM of chronic bronchitis patients by inhibiting LPS induced intracellular calcium ion elevation in AM, suggesting that ASI may inhibit non specific inflammation of respiratory tract.