华南地区HGV的流行状况和同源性分析

Prevalence of HGV infection and homology of different HGV strains in Southern China

目的了解华南地区庚型肝炎病毒（ＨＧＶ）的流行状况及ＨＧＶ不同株和不同基因区的同源性。方法采用逆转录聚合酶链反应技术（ＲＴ－ＰＣＲ）检测来自广东、香港、云南的不同人群血清标本共１９９１份。对其中２０份的５端非编码区（５ＵＴＲ）２３８ｂｐ和３份非结构蛋白５区（ＮＳ５）６２１ｂｐ进行了序列测定和同源性分析。结果一般人群ＨＧＶＲＮＡ阳性率为（０．７３～１．３４）％，献血员（２．５２～２．９０）％，静脉吸毒者１７．８６％，血液透析患者１４．１３％，接受骨髓移植者４１．６７％，在非甲～戊型肝炎病人为２５．３０％，肝细胞癌１４．４８％，乙型肝炎７．２２％，丙型肝炎（８．３３～１６．１３）％。不同株５ＵＴＲ同源性介乎（９０．４０～１００）％；不同株ＮＳ５区核苷酸同源性（９３．３０～９４．００）％，氨基酸为（９７～９９．２）％。结论接受骨髓移植、血液透析、静脉吸毒者，乙型、丙型、非甲～戊型肝炎病人及肝细胞癌患者是ＨＧＶ的高感染人群。不同ＨＧＶ株存在一定的地区性差异；同一地区不同人群的ＨＧＶ株变异不明显；

Objectives To investigate the prevalence of hepatitis G virus (HGV) infection and to analyse the homology of different HGV strains in Southern China. Methods A total of 1991 sera from different groups in Guangdong, Hong Kong and Yunnan were detected by reverse transcription polymerase chain reaction (RT PCR). The nucleotide sequences of 5' untranslated region (5' UTR) derived from 20 strains and NS5 region from 3 strains were determined. Results The positive rate of HGV RNA was 0.78% 1.34% in society population, 2.52% 2.90% in blood donors, 17.86% in intravenous drug users (IVDUs), 14.34% in patients with hemodialysis, 14.48% in patients with hepatocellular carcinoma, 25.30% in non A E hepatitis, 7.22% in hepatitis B, 8.33% 16.13% in hepatitis C, 41.67% in patients with bone marrow transplantation (BMT), respectively. The homology was 90.40% 100% in 5' UTR between different strains. The homology of NS5 region was 93.3% 94% in nucleotide sequence, 97% 99.2% in amino acid sequence. Conclusions The HGV infection rates among populations having accepted bone marrow transplantation, hemodialysis, and patients with hepatitis B, non A E hepatitis, hepatitis C and hepatocellular carcinoma as well as the intravenous drug abusers were high. Sequence variation in different strains were associated with geographical factors. No apparent variation between different strains from different groups in Southern China and no mutation in same patient over a period of 3 months were observed. Some highly conserved and relatively variable areas were seen.