巴戟天醇提物对D-半乳糖致衰老大鼠小脑的作用

Effects of BEM on Cerebellum of D-galactose Induced Aged Rat

目的:观察巴戟天醇提物对D-半乳糖致衰老大鼠小脑的作用。方法:6月龄Wistar大鼠80只,雌雄各半,随机分为空白对照组、衰老模型组、阳性对照药(维生素E100mg/kg)组、巴戟天醇提物2.1g/kg和0.7g/kg两个剂量组,共5组,每组16只。各组一半动物,用分光光度计测定大鼠小脑组织中的SOD活性及MDA含量,以此分析巴戟天的抗氧自由基作用;另一半动物采用甲基绿-哌洛宁染色法观察大鼠小脑皮质蒲肯野细胞的形态变化,计算凋亡指数,用免疫组化法检测bcl-2蛋白及bax蛋白表达情况,以此来观察巴戟天醇提物的抗凋亡作用及其机制。结果:与空白对照组比较,衰老模型组大鼠小脑组织超氧化物歧化酶(SOD)活性降低、丙二醛(MDA)含量增加、蒲肯野细胞的凋亡指数增、bcl-2蛋白表达显著降低、bax蛋白表达显著升高(P<0.01);维生素E100mg/kg组、巴戟天醇提物2.1g/kg和0.7g/kg两个剂量组与衰老模型组相比,小脑组织SOD活性均明显增加、MDA含量显著降低、蒲肯野细胞凋亡指数显著降低、bcl-2蛋白表达显著升高、bax蛋白表达显著降低(P<0.01)。巴戟天醇提物2.1g/kg组与VitE100mg/kg组的作用差异无显著性(P>0.05),而巴戟天醇提物0.7g/kg组与前两组相比小脑组织SOD活性降低、MDA含量增加、蒲肯野细胞的凋亡指数增、bcl-2蛋白表达显著降低、bax蛋白表达显著升高(P<0.05)。结论:巴戟天醇提物对D-半乳糖致衰老大鼠小脑组织具有抗氧化作用及抗凋亡作用;巴戟天醇提物的抗凋亡作用可能与bcl-2pro-tein和bax蛋白表达的变化有关;巴戟天醇提物的抗氧化及抗细胞凋亡作用与阳性对照药VitE相似。

Objective：Model of D-galactose induced aged rats was established to observe how BEM effect SOD vigour,MDA density,the apoptotic index of purkinje fibers,Bcl-2 protein and Bax protein in cerebellum of D-galactose induced aged rat,and Vitamine E（VE） was used as positive control.Methods：80 healthy wistar rats which were 6 months,were applied in this study and divided into 5 groups at random：normal group,aging model group,positive control（Vit E 100 mg/kg） group,two BEM groups with the dose of 2.1 g/kg and 0.7 g/kg,and each group had 16 rats.In each group,one half of the animals were detected SOD activities and the content of MDA;the other half were detect the apoptotic index of purkinje fibers of cerebellum cortex of rats in each group by methy/green pyronin stain and Bcl-2 protein and Bax protein by immunohistochemical method.Results：Comparing the aging group with the normal group,there is significant decrease in SOD vigour,significant increase in the content of MDA,significant decrease in apoptotic index of purkinje fibers,significant decrease in Bcl-2 protein and significant increase Bax protein（P〈0.01）.Comparing two BEM groups with the dose of 2.1 g/kg and 0.7 g/kg and VE 100.0 mg/kg group with the normal group,there is significant increase in SOD vigour,significant decrease in the content of MDA,significant decrease in apoptotic index of purkinje fibers,significant increase in Bcl-2 protein and significant decrease Bax protein（P〈0.01）.The difference of the effects between BEM group with the dose of 2.1 g/kg and VE 100.0 mg/kg group is insignificant（P〉0.05）.comparing BEM group with dose of 0.7 g/kg with the former two groups,there is significant decrease in SOD vigour,significant increase in the content of MDA,significant decrease in apoptotic index of purkinje fibers,significant decrease in Bcl-2 protein and significant increase Bax protein（P〈0.05）.Conclusion：BEM has the anti-oxidative and anti-apoptotic effects;The anti-apoptotic effect is relative with significant increase in Bcl-2 protein and significant decrease Bax protein;The anti-oxidative and anti-apoptotic effects of DIE is similar to VE.