霍乱弧菌O_(139)菌毛微球疫苗制备初探

Vibrio Cholerae O 139 Pili Microsphere Vaccine Research

为寻求 Ｏ１３９ 型霍乱弧菌菌毛疫苗研制的新途径， 用高分子聚乳酸－ 聚乙二醇共聚物（ Ｄ Ｌ－ Ｐ Ｌ Ｇ） 包裹毒素共调菌毛（ Ｔ Ｃ Ｐ） 抗原， 进行免疫学研究． 结果表明微球疫苗比游离疫苗诱导的抗体水平高， 其中小鼠血清 Ｉｇ Ｇ 以皮下 Ｍ Ｓ－ Ｔ Ｃ Ｐ 组最高（１∶７ ６８０） ， 唾液中的ｓ Ｉｇ Ａ 以口服 Ｍ Ｓ－ Ｔ Ｃ Ｐ 组最高（１ ： ６０） ． 口服组主要诱导粘膜免疫， 皮下组主要诱导全身性免疫． Ｔ Ｃ Ｐ 是霍乱弧菌的共同抗原之一， 可作为霍乱疫苗的候选抗原．

To provide the base of manufacturing Vibrio cholera O 139 subunit vaccine, toxin-coregulated pili (TCP) was encapsulated in Poly-DL-lactide-Poly ethylene glycol microspheres, which were distributed over iver, spleen tissues after mice were immunized orally for four weeks. Sera IgG and salivary sIgA after immunization were assayed by BA-ELISA and the results showed:(1) IgG titer of subcutaneous-MS-TCP injection group was the highest among the test groups. (2) sIgA titer of oral-MS-TCP group was the highest among the test groups. These results suggested that mucosal immune responses were mainly induced in oral immunization groups and systemic immune responses were chiefly induced in subcutaneous injection groups. TCP was one of the common antigens of Vibrio cholera, and could be a candidate antigen of cholera vaccine.