DMBA诱发金黄地鼠颊粘膜癌及肉瘤形成的观察

Induction of Carcinoma and Sarcoma in Hamster Buccal Pouch with dimethylbenaxthcene

为探讨化学致癌剂诱导实验性口腔癌发生过程中结缔组织改变及其肿瘤起源和意义． 年轻金黄地鼠， 用０５ ％ Ｄ Ｍ Ｂ Ａ （ 二甲基苯并蒽） 丙酮液处理其右侧颊囊， 对照组不处理或仅纯丙酮液处理． ３ ， ６ ，９ ，１２ 和１６ 周分批处死动物， 观察诱癌区病理学改变； 拟有结缔组织恶变者， 用抗ｋｅｒａｔｉｎ ， ｖｉｍｅｎｔｉｎ 和ｄｅｓｍｉｎ 抗体进行免疫组化染色 （ Ｌ Ｓ Ａ Ｂ 法） ． 结果表明 Ｄ Ｍ Ｂ Ａ 作用于地鼠颊粘膜， 不仅可诱发上皮癌变， 而且结缔组织也发生一序列改变： 纤维细胞增殖， 血管增生和炎性细胞浸润等， 甚至少数（８／３０ 例） 动物在异常增生上皮下方或癌巢周围出现类似纤维肉瘤或梭形鳞癌的肿瘤细胞， 免疫组化证实为来自间叶组织的纤维肉瘤． 本研究结果提示化学致癌剂致癌过程可能存在上皮结缔组织相互作用， 此将有助于提高对口腔癌发生生物学及防治策略的认识．

To investigate the sequential histopathological changes for both epithelium and connective tissue during chemical carcinogen induced experimental oral carcinoma, 0.5% DMBA in acetone was applied topically to hamster right buccal pouch three times per week. The control received no treatment or pure acetone. These hamsters were sacrificed after 3,6,9,12 and 16 weeks. Some malignant tumors developed in the underlying connective tissue were differentiated by immunohistochemically staining. Results: DMBA induced ont lnly epithelium malignant transformation, but also a series of abnormal changes underlying connective tissue including proliferation of fibroblasts, angiogenesis and inflammatory cell infiltration. The result showed a disturbance induced by chemical carcinogen in epithelial-mesenchymal interactions might be important in understanding the genestic biology and improving the treatment of oral mucosa precancerous lesion and cancer.